4OI9

Crystal Structure of ICAM-5 D1-D4 ectodomain fragment, Space Group P21

4OI9 の概要

• エントリーDOI: 10.2210/pdb4oi9/pdb
• 関連するPDBエントリー: 4OIA 4OIB
• 分子名称: Intercellular adhesion molecule 5, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: cell adhesion, cell surface, neurons, immunoglobulin superfamily, intercellular adhesion molecules, icam-5, telencephalin
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: Q9UMF0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46363.69

構造登録者

Recacha, R.,Jimenez, D.,Tian, L.,Barredo, R.,Ghamberg, C.,Casasnovas, J.M. (登録日: 2014-01-19, 公開日: 2014-07-16, 最終更新日: 2024-11-20)

主引用文献

Recacha, R.,Jimenez, D.,Tian, L.,Barredo, R.,Gahmberg, C.G.,Casasnovas, J.M.
Crystal structures of an ICAM-5 ectodomain fragment show electrostatic-based homophilic adhesions.
Acta Crystallogr.,Sect.D, 70:1934-1943, 2014

PubMed Abstract: Intercellular cell adhesion molecule-5 (ICAM-5) is a member of the ICAM subfamily that is exclusively expressed in the telencephalon region of the brain. The crystal structure of the four most N-terminal glycosylated domains (D1-D4) of ICAM-5 was determined in three different space groups and the D1-D5 fragment was modelled. The structures showed a curved molecule with two pronounced interdomain bends between D2 and D3 and between D3 and D4, as well as some interdomain flexibility. In contrast to ICAM-1, ICAM-5 has patches of positive and negative electrostatic charge at D1-D2 and at D3-D5, respectively. ICAM-5 can mediate homotypic interactions. In the crystals, several charge-based intermolecular interactions between the N-terminal and C-terminal moieties of the ICAM-5 molecules were observed, which defined an interacting surface in the D1-D4 fragment. One of the crystal lattices has a molecular assembly that could represent the homophilic ICAM-5 cell adhesion complex in neurons.

PubMed: 25004970
DOI: 10.1107/S1399004714009468

実験手法

X-RAY DIFFRACTION (2.5 Å)