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4OI6

Crystal structure analysis of nickel-bound form SCO4226 from Streptomyces coelicolor A3(2)

4OI6 の概要
エントリーDOI10.2210/pdb4oi6/pdb
関連するPDBエントリー4OI3
分子名称Nickel responsive protein, NICKEL (II) ION, CITRIC ACID, ... (4 entities in total)
機能のキーワードnickel responsive protein, structural genomics, ferredoxin-like fold, a nickel responsive protein, nickel binding, metal binding protein
由来する生物種Streptomyces coelicolor
タンパク質・核酸の鎖数2
化学式量合計20850.10
構造登録者
Lu, M.,Jiang, Y.L.,Wang, S.,Cheng, W.,Zhang, R.G.,Virolle, M.J.,Chen, Y.,Zhou, C.Z. (登録日: 2014-01-18, 公開日: 2014-09-10, 最終更新日: 2024-10-16)
主引用文献Lu, M.,Jiang, Y.L.,Wang, S.,Jin, H.,Zhang, R.G.,Virolle, M.J.,Chen, Y.,Zhou, C.Z.
Streptomyces coelicolor SCO4226 Is a Nickel Binding Protein.
Plos One, 9:e109660-e109660, 2014
Cited by
PubMed Abstract: The open reading frame SCO4226 of Streptomyces coelicolor A3(2) encodes an 82-residue hypothetical protein. Biochemical assays revealed that each SCO4226 dimer binds four nickel ions. To decipher the molecular function, we solved the crystal structures of SCO4226 in both apo- and nickel-bound (Ni-SCO4226) forms at 1.30 and 2.04 Å resolution, respectively. Each subunit of SCO4226 dimer adopts a canonical ferredoxin-like fold with five β-strands flanked by two α-helices. In the structure of Ni-SCO4226, four nickel ions are coordinated at the surface of the dimer. Further biochemical assays suggested that the binding of Ni2+ triggers the self-aggregation of SCO4226 in vitro. In addition, RT-qPCR assays demonstrated that the expression of SCO4226 gene in S. coelicolor is specifically up-regulated by the addition of Ni2+, but not other divalent ions such as Cu2+, Mn2+ or Co2+. All these results suggested that SCO4226 acts as a nickel binding protein, probably required for nickel sequestration and/or detoxification.
PubMed: 25285530
DOI: 10.1371/journal.pone.0109660
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.04 Å)
構造検証レポート
Validation report summary of 4oi6
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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