4OFT

C- Orthorombic NaGST1

4OFT の概要

• エントリーDOI: 10.2210/pdb4oft/pdb
• 関連するPDBエントリー: 2ON7 4OFM 4OFN
• 分子名称: Glutathione S-transferase-1 (2 entities in total)
• 機能のキーワード: gst, transferase
• 由来する生物種: Necator americanus (Human hookworm)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47420.72

構造登録者

Asojo, O.A. (登録日: 2014-01-15, 公開日: 2014-12-03, 最終更新日: 2024-11-06)

主引用文献

Asojo, O.A.,Ceccarelli, C.
Structure of glutathione S-transferase 1 from the major human hookworm parasite Necator americanus (Na-GST-1) in complex with glutathione.
Acta Crystallogr F Struct Biol Commun, 70:1162-1166, 2014

PubMed Abstract: Glutathione S-transferase 1 from Necator americanus (Na-GST-1) is a vaccine candidate for hookworm infection that has a high affinity for heme and metal porphyrins. As part of attempts to clarify the mechanism of heme detoxification by hookworm GSTs, co-crystallization and soaking studies of Na-GST-1 with the heme-like molecules protoporphyrin IX disodium salt, hematin and zinc protoporphyrin were undertaken. While these studies did not yield the structure of the complex of Na-GST-1 with any of these molecules, co-crystallization experiments resulted in the first structures of the complex of Na-GST-1 with the substrate glutathione. The structures of the complex of Na-GST-1 with glutathione were solved from pathological crystalline aggregates comprising more than one crystal form. These first structures of the complex of Na-GST-1 with the substrate glutathione were solved by molecular replacement from data collected with a sealed-tube home source using the previously reported apo structure as the search model.

PubMed: 25195885
DOI: 10.1107/S2053230X1401646X

実験手法

X-RAY DIFFRACTION (2.6 Å)