4OFD

Crystal Structure of mouse Neph1 D1-D2

4OFD の概要

• エントリーDOI: 10.2210/pdb4ofd/pdb
• 関連するPDBエントリー: 4OF0 4OF3 4OF6 4OF7 4OF8 4OFI 4OFK 4OFP 4OFY
• 分子名称: Kin of IRRE-like protein 1, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: immunoglobulin superfamily, cell adhesion, kidney, blood filtration, protein binding, n-linked glycosylation, signaling protein, membrane, extracellular
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47891.57

構造登録者

Ozkan, E.,Garcia, K.C. (登録日: 2014-01-14, 公開日: 2014-02-19, 最終更新日: 2024-10-30)

主引用文献

Ozkan, E.,Chia, P.H.,Wang, R.R.,Goriatcheva, N.,Borek, D.,Otwinowski, Z.,Walz, T.,Shen, K.,Garcia, K.C.
Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis.
Cell(Cambridge,Mass.), 156:482-494, 2014

PubMed Abstract: SYG-1 and SYG-2 are multipurpose cell adhesion molecules (CAMs) that have evolved across all major animal taxa to participate in diverse physiological functions, ranging from synapse formation to formation of the kidney filtration barrier. In the crystal structures of several SYG-1 and SYG-2 orthologs and their complexes, we find that SYG-1 orthologs homodimerize through a common, bispecific interface that similarly mediates an unusual orthogonal docking geometry in the heterophilic SYG-1/SYG-2 complex. C. elegans SYG-1's specification of proper synapse formation in vivo closely correlates with the heterophilic complex affinity, which appears to be tuned for optimal function. Furthermore, replacement of the interacting domains of SYG-1 and SYG-2 with those from CAM complexes that assume alternative docking geometries or the introduction of segmental flexibility compromised synaptic function. These results suggest that SYG extracellular complexes do not simply act as "molecular velcro" and that their distinct structural features are important in instructing synaptogenesis. PAPERFLICK:

PubMed: 24485456
DOI: 10.1016/j.cell.2014.01.004

実験手法

X-RAY DIFFRACTION (3.94 Å)