4O8J
Crystal structure of RtcA, the RNA 3'-terminal phosphate cyclase from Pyrococcus horikoshii, in complex with rACAAA3'phosphate and adenine.
Summary for 4O8J
| Entry DOI | 10.2210/pdb4o8j/pdb |
| Related | 4O89 |
| Descriptor | RNA 3'-terminal phosphate cyclase, RNA, ADENOSINE, ... (5 entities in total) |
| Functional Keywords | 3' rna cyclase, rna, ligase-rna complex, ligase/rna |
| Biological source | Pyrococcus horikoshii More |
| Total number of polymer chains | 4 |
| Total formula weight | 79027.46 |
| Authors | Desai, K.K.,Bingman, C.A.,Phillips Jr., G.N.,Raines, R.T. (deposition date: 2013-12-27, release date: 2014-09-10, Last modification date: 2024-10-16) |
| Primary citation | Desai, K.K.,Bingman, C.A.,Cheng, C.L.,Phillips Jr., G.N.,Raines, R.T. Structure of RNA 3'-phosphate cyclase bound to substrate RNA. Rna, 20:1560-1566, 2014 Cited by PubMed Abstract: RNA 3'-phosphate cyclase (RtcA) catalyzes the ATP-dependent cyclization of a 3'-phosphate to form a 2',3'-cyclic phosphate at RNA termini. Cyclization proceeds through RtcA-AMP and RNA(3')pp(5')A covalent intermediates, which are analogous to intermediates formed during catalysis by the tRNA ligase RtcB. Here we present a crystal structure of Pyrococcus horikoshii RtcA in complex with a 3'-phosphate terminated RNA and adenosine in the AMP-binding pocket. Our data reveal that RtcA recognizes substrate RNA by ensuring that the terminal 3'-phosphate makes a large contribution to RNA binding. Furthermore, the RNA 3'-phosphate is poised for in-line attack on the P-N bond that links the phosphorous atom of AMP to N(ε) of His307. Thus, we provide the first insights into RNA 3'-phosphate termini recognition and the mechanism of 3'-phosphate activation by an Rtc enzyme. PubMed: 25161314DOI: 10.1261/rna.045823.114 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.04 Å) |
Structure validation
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