4O6P

Structural and functional studies the characterization of C58G/C70G mutant in Cys4 Zinc-finger motif in the recombination mediator protein RecR

4O6P の概要

• エントリーDOI: 10.2210/pdb4o6p/pdb
• 関連するPDBエントリー: 4O6O
• 分子名称: Recombination protein RecR, ZINC ION (3 entities in total)
• 機能のキーワード: zinc finger, dna repair, dna binding, recombination
• 由来する生物種: Thermoanaerobacter tengcongensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47217.00

構造登録者

Tang, Q.,Liu, Y.P.,Yan, X.X.,Liang, D.C. (登録日: 2013-12-23, 公開日: 2014-12-10, 最終更新日: 2023-11-08)

主引用文献

Tang, Q.,Liu, Y.P.,Yan, X.X.,Liang, D.C.
Structural and functional characterization of Cys4 zinc finger motif in the recombination mediator protein RecR.
DNA Repair (Amst.), 24:10-14, 2014

PubMed Abstract: Zinc finger motif widely exists in protein structure, which can play different roles in different proteins. RecR is an important recombination mediator protein (RMP) in the RecFOR pathway and zinc finger motif is the most conserved domain in RecR protein. However, the function of this zinc finger motif in RecR is unclear. Here, we have studied the structures of the single cysteine and double cysteines mutation within the zinc finger motif in Thermoanaerobacter tengcongensis RecR (TTERecR). We have also studied the DNA binding ability as well as TTERecO protein binding ability of single, double and even triple cysteines mutation of the zinc finger motif, and the mutants do not alter DNA binding by RecR nor the interaction between RecR and RecO. The function of TTERecR zinc finger motif is to maintain the stability of the three-dimensional structure.

PubMed: 25460918
DOI: 10.1016/j.dnarep.2014.09.012

実験手法

X-RAY DIFFRACTION (3 Å)