4O41

Amide linked RNA

4O41 の概要

• エントリーDOI: 10.2210/pdb4o41/pdb
• 分子名称: AMIDE LINKED RNA, STRONTIUM ION (3 entities in total)
• 機能のキーワード: modified rna, rna structure, amide rna, amide internucleoside linkage, modified nucleoside, rna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 16461.38

構造登録者

Pallan, P.S.,Egli, M. (登録日: 2013-12-18, 公開日: 2014-07-09, 最終更新日: 2024-02-28)

主引用文献

Mutisya, D.,Selvam, C.,Lunstad, B.D.,Pallan, P.S.,Haas, A.,Leake, D.,Egli, M.,Rozners, E.
Amides are excellent mimics of phosphate internucleoside linkages and are well tolerated in short interfering RNAs.
Nucleic Acids Res., 42:6542-6551, 2014

PubMed Abstract: RNA interference (RNAi) has become an important tool in functional genomics and has an intriguing therapeutic potential. However, the current design of short interfering RNAs (siRNAs) is not optimal for in vivo applications. Non-ionic phosphate backbone modifications may have the potential to improve the properties of siRNAs, but are little explored in RNAi technologies. Using X-ray crystallography and RNAi activity assays, the present study demonstrates that 3'-CH2-CO-NH-5' amides are excellent replacements for phosphodiester internucleoside linkages in RNA. The crystal structure shows that amide-modified RNA forms a typical A-form duplex. The amide carbonyl group points into the major groove and assumes an orientation that is similar to the P-OP2 bond in the phosphate linkage. Amide linkages are well hydrated by tandem waters linking the carbonyl group and adjacent phosphate oxygens. Amides are tolerated at internal positions of both the guide and passenger strand of siRNAs and may increase the silencing activity when placed near the 5'-end of the passenger strand. As a result, an siRNA containing eight amide linkages is more active than the unmodified control. The results suggest that RNAi may tolerate even more extensive amide modification, which may be useful for optimization of siRNAs for in vivo applications.

PubMed: 24813446
DOI: 10.1093/nar/gku235

実験手法

X-RAY DIFFRACTION (1.2 Å)