4O01

Crystal Structure of D. radiodurans Bacteriophytochrome Photosensory Core Module in its Illuminated Form

4O01 の概要

• エントリーDOI: 10.2210/pdb4o01/pdb
• 関連するPDBエントリー: 4O0P
• 分子名称: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid (2 entities in total)
• 機能のキーワード: phytochrome, red-light photoreceptor, photoconversion, chromophore, sensory transduction, signaling protein, biliverdin, transferase
• 由来する生物種: Deinococcus radiodurans R1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 228666.79

構造登録者

Takala, H.,Ihalainen, J.A.,Westenhoff, S. (登録日: 2013-12-13, 公開日: 2014-05-07, 最終更新日: 2024-10-30)

主引用文献

Takala, H.,Bjorling, A.,Berntsson, O.,Lehtivuori, H.,Niebling, S.,Hoernke, M.,Kosheleva, I.,Henning, R.,Menzel, A.,Ihalainen, J.A.,Westenhoff, S.
Signal amplification and transduction in phytochrome photosensors
Nature, 509:245-248, 2014

PubMed Abstract: Sensory proteins must relay structural signals from the sensory site over large distances to regulatory output domains. Phytochromes are a major family of red-light-sensing kinases that control diverse cellular functions in plants, bacteria and fungi. Bacterial phytochromes consist of a photosensory core and a carboxy-terminal regulatory domain. Structures of photosensory cores are reported in the resting state and conformational responses to light activation have been proposed in the vicinity of the chromophore. However, the structure of the signalling state and the mechanism of downstream signal relay through the photosensory core remain elusive. Here we report crystal and solution structures of the resting and activated states of the photosensory core of the bacteriophytochrome from Deinococcus radiodurans. The structures show an open and closed form of the dimeric protein for the activated and resting states, respectively. This nanometre-scale rearrangement is controlled by refolding of an evolutionarily conserved 'tongue', which is in contact with the chromophore. The findings reveal an unusual mechanism in which atomic-scale conformational changes around the chromophore are first amplified into an ångstrom-scale distance change in the tongue, and further grow into a nanometre-scale conformational signal. The structural mechanism is a blueprint for understanding how phytochromes connect to the cellular signalling network.

PubMed: 24776794
DOI: 10.1038/nature13310

実験手法

X-RAY DIFFRACTION (3.24 Å)