4NY6

Neutron structure of leucine and valine methyl protonated type III antifreeze

4NY6 の概要

• エントリーDOI: 10.2210/pdb4ny6/pdb
• 関連するPDBエントリー: 3QF6
• 分子名称: Type-3 ice-structuring protein HPLC 12 (2 entities in total)
• 機能のキーワード: antifreeze protein
• 由来する生物種: Zoarces americanus (North-Atlantic ocean pout,mother-of-eels,muttonfish,poodler)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6894.13

構造登録者

Fisher, S.J.,Blakeley, M.P.,Howard, E.I.,Petite-Haertlein, I.,Haertlein, M.,Mitschler, A.,Cousido-Siah, A.,Salvaya, A.G.,Popov, A.,Muller-Dieckmann, C.,Petrova, T.,Podjarny, A.D. (登録日: 2013-12-10, 公開日: 2014-12-24, 最終更新日: 2024-02-28)

主引用文献

Fisher, S.J.,Blakeley, M.P.,Howard, E.I.,Petit-Haertlein, I.,Haertlein, M.,Mitschler, A.,Cousido-Siah, A.,Salvay, A.G.,Popov, A.,Muller-Dieckmann, C.,Petrova, T.,Podjarny, A.
Perdeuteration: improved visualization of solvent structure in neutron macromolecular crystallography.
Acta Crystallogr.,Sect.D, 70:3266-3272, 2014

PubMed Abstract: The 1.8 Å resolution neutron structure of deuterated type III antifreeze protein in which the methyl groups of leucine and valine residues are selectively protonated is presented. Comparison between this and the 1.85 Å resolution neutron structure of perdeuterated type III antifreeze protein indicates that perdeuteration improves the visibility of solvent molecules located in close vicinity to hydrophobic residues, as cancellation effects between H atoms of the methyl groups and nearby heavy-water molecules (D2O) are avoided.

PubMed: 25478844
DOI: 10.1107/S1399004714021610

実験手法

NEUTRON DIFFRACTION (1.85 Å)
X-RAY DIFFRACTION (1.05 Å)