4NWP

Computationally Designed Two-Component Self-Assembling Tetrahedral Cage, T33-21, Crystallized in Space Group R32

4NWP の概要

• エントリーDOI: 10.2210/pdb4nwp/pdb
• 関連するPDBエントリー: 4NWN 4NWO 4NWQ 4NWR
• 分子名称: Putative uncharacterized protein, Uncharacterized protein, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: two-component, self-assembling, tetrahedron, designed protein cage, computational design, computational biology, protein engineering, multimerization, nanomaterial, nanostructure, protein binding
• 由来する生物種: Pyrococcus horikoshii; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 137359.23

構造登録者

McNamara, D.E.,King, N.P.,Bale, J.B.,Sheffler, W.,Baker, D.,Yeates, T.O. (登録日: 2013-12-06, 公開日: 2014-05-28, 最終更新日: 2023-09-20)

主引用文献

King, N.P.,Bale, J.B.,Sheffler, W.,McNamara, D.E.,Gonen, S.,Gonen, T.,Yeates, T.O.,Baker, D.
Accurate design of co-assembling multi-component protein nanomaterials.
Nature, 510:103-108, 2014

PubMed Abstract: The self-assembly of proteins into highly ordered nanoscale architectures is a hallmark of biological systems. The sophisticated functions of these molecular machines have inspired the development of methods to engineer self-assembling protein nanostructures; however, the design of multi-component protein nanomaterials with high accuracy remains an outstanding challenge. Here we report a computational method for designing protein nanomaterials in which multiple copies of two distinct subunits co-assemble into a specific architecture. We use the method to design five 24-subunit cage-like protein nanomaterials in two distinct symmetric architectures and experimentally demonstrate that their structures are in close agreement with the computational design models. The accuracy of the method and the number and variety of two-component materials that it makes accessible suggest a route to the construction of functional protein nanomaterials tailored to specific applications.

PubMed: 24870237
DOI: 10.1038/nature13404

実験手法

X-RAY DIFFRACTION (2.1 Å)