4NS3

Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD and cobalamin

4NS3 の概要

• エントリーDOI: 10.2210/pdb4ns3/pdb
• 関連するPDBエントリー: 4LEM
• 分子名称: Delta-1-pyrroline-5-carboxylate dehydrogenase, COBALAMIN, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: rossmann fold, dehydrogenase, oxidoreductase, dehydrogenation
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 373912.42

構造登録者

Lagautriere, T.,Bashiri, G.,Baker, E.N. (登録日: 2013-11-27, 公開日: 2015-01-14, 最終更新日: 2024-10-16)

主引用文献

Lagautriere, T.,Bashiri, G.,Baker, E.N.
Use of a "silver bullet" to resolve crystal lattice dislocation disorder: A cobalamin complex of Delta (1)-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis.
J.Struct.Biol., 189:153-157, 2015

PubMed Abstract: The use of small molecules as "silver bullets" that can bind to generate crosslinks between protein molecules has been advanced as a powerful means of enhancing success in protein crystallization (McPherson and Cudney, 2006). We have explored this approach in attempts to overcome an order-disorder phenomenon that complicated the structural analysis of the enzyme Δ(1)-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis (P5CDH, Mtb-PruA). Using the Silver Bullets Bio screen, we obtained new crystal packing using cobalamin as a co-crystallization agent. This crystal form did not display the order-disorder phenomenon previously encountered. Solution of the crystal structure showed that cobalamin molecules are present in the crystal contacts. Although the cobalamin binding probably does not have physiological relevance, it reflects similarities in the nucleotide-binding region of Mtb-PruA, with the nucleotide loop of cobalamin sharing the binding site for the adenine moiety of NAD(+).

PubMed: 25557497
DOI: 10.1016/j.jsb.2014.12.007

実験手法

X-RAY DIFFRACTION (2.38 Å)