4NQX

Crystal Structure of HLA A*0101 in complex with NP44-S7N, an 9-mer influenza epitope

4NQX の概要

• エントリーDOI: 10.2210/pdb4nqx/pdb
• 関連するPDBエントリー: 4NQV
• 分子名称: HLA class I histocompatibility antigen, A-1 alpha chain, Beta-2-microglobulin, NP44-S7N mutant peptide, CTELKLNDY, ... (4 entities in total)
• 機能のキーワード: immune system, presenting viral epitope, t cell receptor, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P30443; Secreted: P61769
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 274090.48

構造登録者

Rossjohn, J.,Gras, S. (登録日: 2013-11-26, 公開日: 2013-12-25, 最終更新日: 2023-09-20)

主引用文献

Quinones-Parra, S.,Grant, E.,Loh, L.,Nguyen, T.H.,Campbell, K.A.,Tong, S.Y.,Miller, A.,Doherty, P.C.,Vijaykrishna, D.,Rossjohn, J.,Gras, S.,Kedzierska, K.
Preexisting CD8+ T-cell immunity to the H7N9 influenza A virus varies across ethnicities.
Proc.Natl.Acad.Sci.USA, 111:1049-1054, 2014

PubMed Abstract: The absence of preexisting neutralizing antibodies specific for the novel A (H7N9) influenza virus indicates a lack of prior human exposure. As influenza A virus-specific CD8(+) T lymphocytes (CTLs) can be broadly cross-reactive, we tested whether immunogenic peptides derived from H7N9 might be recognized by memory CTLs established following infection with other influenza strains. Probing across multiple ethnicities, we identified 32 conserved epitopes derived from the nucleoprotein (NP) and matrix-1 (M1) proteins. These NP and M1 peptides are presented by HLAs prevalent in 16-57% of individuals. Remarkably, some HLA alleles (A*0201, A*0301, B*5701, B*1801, and B*0801) elicit robust CTL responses against any human influenza A virus, including H7N9, whereas ethnicities where HLA-A*0101, A*6801, B*1501, and A*2402 are prominent, show limited CTL response profiles. By this criterion, some groups, especially the Alaskan and Australian Indigenous peoples, would be particularly vulnerable to H7N9 infection. This dissection of CTL-mediated immunity to H7N9 thus suggests strategies for both vaccine delivery and development.

PubMed: 24395804
DOI: 10.1073/pnas.1322229111

実験手法

X-RAY DIFFRACTION (2 Å)