4NPM

Crystal structure of Zebrafish ALKBH5 in complex with succinic acid

4NPM の概要

• エントリーDOI: 10.2210/pdb4npm/pdb
• 関連するPDBエントリー: 4NPL
• 分子名称: RNA demethylase ALKBH5, MANGANESE (II) ION, SUCCINIC ACID, ... (4 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Danio rerio (leopard danio,zebra danio,zebra fish)
• 細胞内の位置: Nucleus speckle (By similarity): Q08BA6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57056.90

構造登録者

He, C.,Chen, W.,Zhang, L. (登録日: 2013-11-21, 公開日: 2014-02-19, 最終更新日: 2024-02-28)

主引用文献

Chen, W.,Zhang, L.,Zheng, G.,Fu, Y.,Ji, Q.,Liu, F.,Chen, H.,He, C.
Crystal structure of the RNA demethylase ALKBH5 from zebrafish.
Febs Lett., 588:892-898, 2014

PubMed Abstract: ALKBH5, a member of AlkB family proteins, has been reported as a mammalian N(6)-methyladenosine (m(6)A) RNA demethylase. Here we report the crystal structure of zebrafish ALKBH5 (fALKBH5) with the resolution of 1.65Å. Structural superimposition shows that fALKBH5 is comprised of a conserved jelly-roll motif. However, it possesses a loop that interferes potential binding of a duplex nucleic acid substrate, suggesting an important role in substrate selection. In addition, several active site residues are different between the two known m(6)A RNA demethylases, ALKBH5 and FTO, which may result in their slightly different pathways of m(6)A demethylation.

PubMed: 24561204
DOI: 10.1016/j.febslet.2014.02.021

実験手法

X-RAY DIFFRACTION (1.803 Å)