4NOX

Structure of the nine-bladed beta-propeller of eIF3b

4NOX の概要

• エントリーDOI: 10.2210/pdb4nox/pdb
• 分子名称: Eukaryotic translation initiation factor 3 subunit B, CHLORIDE ION (3 entities in total)
• 機能のキーワード: beta propeller, translation
• 由来する生物種: Chaetomium thermophilum
• 細胞内の位置: Cytoplasm (By similarity): G0RXS6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 86504.34

構造登録者

Liu, Y.,Neumann, P.,Kuhle, B.,Monecke, T.,Ficner, R. (登録日: 2013-11-20, 公開日: 2014-09-17, 最終更新日: 2024-03-20)

主引用文献

Liu, Y.,Neumann, P.,Kuhle, B.,Monecke, T.,Schell, S.,Chari, A.,Ficner, R.
Translation initiation factor eIF3b contains a nine-bladed beta-propeller and interacts with the 40S ribosomal subunit
Structure, 22:923-930, 2014

PubMed Abstract: The multisubunit eukaryotic translation initiation factor 3, among which the subunit b (eIF3b) is a major scaffold protein, plays essential roles in protein synthesis. Here, we report the crystal structure of the WD40 domain of Chaetomium thermophilum eIF3b, revealing a nine-bladed β-propeller fold. Sequence analysis indicates that this propeller architecture is common to all eIF3b orthologs. Revisiting the cryoelectron microscopy (cryo-EM) map of the 43S preinitiation complex suggests an interaction of the eIF3b with the 40S ribosomal subunit involving the ribosomal protein S9e and the 18S rRNA. This model is strongly supported by the direct binding of eIF3b to 40S ribosomes and to the isolated ribosomal protein rpS9e in vitro.

PubMed: 24768115
DOI: 10.1016/j.str.2014.03.010

実験手法

X-RAY DIFFRACTION (2.722 Å)