4NKJ

Structure of influenza B virus hemagglutinin at membrane fusion pH

Summary for 4NKJ

• Entry DOI: 10.2210/pdb4nkj/pdb
• Descriptor: Hemagglutinin HA2 (2 entities in total)
• Functional Keywords: ha, viral protein
• Biological source: Influenza B virus
• Cellular location: Virion membrane; Single-pass type I membrane protein: S5DRP1
• Total number of polymer chains: 1
• Total formula weight: 17211.22

Authors

Ni, F.,Chen, X.,Shen, J.,Wang, Q. (deposition date: 2013-11-12, release date: 2014-04-02)

Primary citation

Ni, F.,Chen, X.,Shen, J.,Wang, Q.
Structural insights into the membrane fusion mechanism mediated by influenza virus hemagglutinin.
Biochemistry, 53:846-854, 2014

PubMed Abstract: Membrane fusion is involved in many fundamental cellular processes and entry of enveloped viruses into host cells. Influenza type A virus HA has long served as a paradigm for mechanistic studies of protein-mediated membrane fusion via large-scale structural rearrangements induced by acidic pH. Here we report the newly determined crystal structure of influenza B virus HA2 in the postfusion state. Together with a large number of previously determined prefusion structures of influenza A and B virus HA and a postfusion structure of influenza A/H3N2 HA2, we identified conserved features that are shared between influenza A and B virus HA in the conformational transition and documented substantial differences that likely influence the detailed mechanisms of this process. Further studies are needed to dissect the effects of these and other structural differences in HA conformational changes and influenza pathogenicity and transmission, which may ultimately expedite the discovery of novel anti-influenza fusion inhibitors.

PubMed: 24433110
DOI: 10.1021/bi401525h

Experimental method

X-RAY DIFFRACTION (2.4535 Å)