4NJ1

GCN4-p1 double Val9, 23 to Ile mutant

Summary for 4NJ1

• Entry DOI: 10.2210/pdb4nj1/pdb
• Related: 2ZTA 4DMD 4NIZ 4NJ0 4NJ2
• Descriptor: General control protein GCN4 (2 entities in total)
• Functional Keywords: transcription
• Biological source: Saccharomyces cerevisiae (Baker's yeast)
• Cellular location: Nucleus: P03069
• Total number of polymer chains: 2
• Total formula weight: 8115.53

Authors

Oshaben, K.M.,Horne, W.S. (deposition date: 2013-11-08, release date: 2014-08-20, Last modification date: 2023-09-20)

Primary citation

Oshaben, K.M.,Horne, W.S.
Tuning assembly size in Peptide-based supramolecular polymers by modulation of subunit association affinity.
Biomacromolecules, 15:1436-1442, 2014

PubMed Abstract: Nature uses proteins and nucleic acids to form a wide array of functional architectures, and scientists have found inspiration from these structures in the rational design of synthetic biomaterials. We have recently shown that a modular subunit consisting of two α-helical coiled coil peptides attached at their midpoints by an organic linking group can spontaneously self-assemble in aqueous solution to form a soluble supramolecular polymer. Here we explore the use of coiled-coil association affinity, readily tuned by amino acid sequence, as a means to predictably alter properties of these supramolecular assemblies. A series of dimeric coiled-coil peptide sequences with identical quaternary folded structures but systematically altered folded stability were designed and biophysically characterized. The sequences were cross-linked to generate a series of branched, self-assembling biomacromolecular subunits. A clear relationship is observed between coiled-coil association affinity and apparent hydrodynamic diameter of the supramolecular polymers formed by these subunits. Our results provide a family of soluble supramolecular polymers of tunable size and well-characterized coiled-coil sequences that add to the library of building blocks available for use in the rational design of protein-based supramolecular biomaterials.

PubMed: 24598042
DOI: 10.1021/bm5000423

Experimental method

X-RAY DIFFRACTION (2 Å)