4NFO

Crystal Structure Analysis of the 16mer GCAGACUUAAGUCUGC

4NFO の概要

• エントリーDOI: 10.2210/pdb4nfo/pdb
• 関連するPDBエントリー: 4NFP 4NFQ
• 分子名称: GCAGACUUAAGUCUGC, SPERMINE (3 entities in total)
• 機能のキーワード: rna 16mer oligo, rna
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 15496.58

構造登録者

Beal, P.A.,Fisher, A.J.,Phelps, K.J.,Ibarra-Soza, J.M.,Zheng, Y. (登録日: 2013-10-31, 公開日: 2014-07-02, 最終更新日: 2024-02-28)

主引用文献

Phelps, K.J.,Ibarra-Soza, J.M.,Tran, K.,Fisher, A.J.,Beal, P.A.
Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA.
Acs Chem.Biol., 9:1780-1787, 2014

PubMed Abstract: Ribonucleoside analogues bearing terminal alkynes, including 7-ethynyl-8-aza-7-deazaadenosine (7-EAA), are useful for RNA modification applications. However, although alkyne- and triazole-bearing ribonucleosides are in widespread use, very little information is available on the impact of these modifications on RNA structure. By solving crystal structures for RNA duplexes containing these analogues, we show that, like adenosine, 7-EAA and a triazole derived from 7-EAA base pair with uridine and are well-accommodated within an A-form helix. We show that copper-catalyzed azide/alkyne cycloaddition (CuAAC) reactions with 7-EAA are sensitive to the RNA secondary structure context, with single-stranded sites reacting faster than duplex sites. 7-EAA and its triazole products are recognized in RNA template strands as adenosine by avian myoblastosis virus reverse transcriptase. In addition, 7-EAA in RNA is a substrate for an active site mutant of the RNA editing adenosine deaminase, ADAR2. These studies extend our understanding of the impact of these novel nucleobase analogues and set the stage for their use in probing RNA structure and metabolism.

PubMed: 24896732
DOI: 10.1021/cb500270x

実験手法

X-RAY DIFFRACTION (1.96 Å)