4NF9

Structure of the Knl1/Nsl1 complex

4NF9 の概要

• エントリーDOI: 10.2210/pdb4nf9/pdb
• 分子名称: Protein CASC5, Kinetochore-associated protein NSL1 homolog, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: rwd domain, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: Q8NG31 Q96IY1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 58609.38

構造登録者

Petrovic, A.,Mosalaganti, S.,Keller, J.,Mattiuzzo, M.,Overlack, K.,Wohlgemuth, S.,Pasqualato, S.,Raunser, S.,Musacchio, A. (登録日: 2013-10-31, 公開日: 2014-03-19, 最終更新日: 2024-11-20)

主引用文献

Petrovic, A.,Mosalaganti, S.,Keller, J.,Mattiuzzo, M.,Overlack, K.,Krenn, V.,De Antoni, A.,Wohlgemuth, S.,Cecatiello, V.,Pasqualato, S.,Raunser, S.,Musacchio, A.
Modular Assembly of RWD Domains on the Mis12 Complex Underlies Outer Kinetochore Organization.
Mol.Cell, 53:591-605, 2014

PubMed Abstract: Faithful chromosome segregation is mandatory for cell and organismal viability. Kinetochores, large protein assemblies embedded in centromeric chromatin, establish a mechanical link between chromosomes and spindle microtubules. The KMN network, a conserved 10-subunit kinetochore complex, harbors the microtubule-binding interface. RWD domains in the KMN subunits Spc24 and Spc25 mediate kinetochore targeting of the microtubule-binding subunits by interacting with the Mis12 complex, a KMN subcomplex that tethers directly onto the underlying chromatin layer. Here, we show that Knl1, a KMN subunit involved in mitotic checkpoint signaling, also contains RWD domains that bind the Mis12 complex and that mediate kinetochore targeting of Knl1. By reporting the first 3D electron microscopy structure of the KMN network, we provide a comprehensive framework to interpret how interactions of RWD-containing proteins with the Mis12 complex shape KMN network topology. Our observations unveil a regular pattern in the construction of the outer kinetochore.

PubMed: 24530301
DOI: 10.1016/j.molcel.2014.01.019

実験手法

X-RAY DIFFRACTION (2.8 Å)