4NDW

Crystal STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS ESX-1 SECRETED PROTEIN REGULATOR (EspR)

Summary for 4NDW

• Entry DOI: 10.2210/pdb4ndw/pdb
• Related: 3QF3 3R1F
• Descriptor: Nucleoid-associated protein EspR (1 entity in total)
• Functional Keywords: all helical, helix-turn-helix motif, transactional regulator, dna binding, transcription
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 2
• Total formula weight: 34062.47

Authors

Gangwar, S.P.,Meena, S.R.,Saxena, A.K. (deposition date: 2013-10-28, release date: 2014-04-23, Last modification date: 2024-03-20)

Primary citation

Gangwar, S.P.,Meena, S.R.,Saxena, A.K.
Comparison of four different crystal forms of the Mycobacterium tuberculosis ESX-1 secreted protein regulator EspR
Acta Crystallogr.,Sect.F, 70:433-437, 2014

PubMed Abstract: The Mycobacterium tuberculosis ESX-1 secreted protein regulator (EspR, Rv3849) is the key protein that delivers bacterial proteins into the host cell during mycobacterial infection. EspR binds directly to the espACD operon and is involved in transcriptional activation. In the current study, M. tuberculosis EspR has been crystallized and its X-ray structure has been determined at 3.3 Å resolution in a P3221 crystal form. EspR forms a physiological dimer in the crystal. Each EspR monomer contains an N-terminal helix-turn-helix DNA-binding domain and a C-terminal dimerization domain. The EspR structure in the P3221 crystal form was compared with previously determined EspR structures in P32, P21 and P212121 crystal forms. Structural comparison analysis indicated that the N-terminal helix-turn-helix domain of EspR acquires a rigid structure in the four crystal forms. However, significant structural differences were observed in the C-terminal domain of EspR in the P21 crystal form when compared with the P3221 and P32 crystal forms. The interaction, stabilization energy and buried surface area analysis of EspR in the four different crystal forms have provided information about the physiological dimer interface of EspR.

PubMed: 24699733
DOI: 10.1107/S2053230X14004166

Experimental method

X-RAY DIFFRACTION (3.3 Å)