4NDP

Crystal structure Molybdenum Storage Protein with fully Mo-loaded cavity

4NDP の概要

• エントリーDOI: 10.2210/pdb4ndp/pdb
• 関連するPDBエントリー: 2OGX 4F6T 4NDO 4NDP 4NDQ 4NDR
• 分子名称: Molybdenum storage protein subunit beta, Molybdenum storage protein subunit alpha, ADENOSINE-5'-TRIPHOSPHATE, ... (9 entities in total)
• 機能のキーワード: rossmann fold, molybdenum storage, atp binding, molybdenum binding, metal binding protein
• 由来する生物種: Azotobacter vinelandii; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63830.74

構造登録者

Poppe, J.,Warkentin, E.,Demmer, U.,Ermler, U. (登録日: 2013-10-27, 公開日: 2014-08-13, 最終更新日: 2023-09-20)

主引用文献

Poppe, J.,Warkentin, E.,Demmer, U.,Kowalewski, B.,Dierks, T.,Schneider, K.,Ermler, U.
Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein.
J.Inorg.Biochem., 138:122-128, 2014

PubMed Abstract: The molybdenum storage protein (MoSto) can store more than 100 Mo or W atoms as discrete polyoxometalate (POM) clusters. Here, we describe the three POM cluster sites along the threefold axis of the protein complex based on four X-ray structures with slightly different polyoxomolybdate compositions between 1.35 and 2 Å resolution. In contrast to the Moα-out binding site occupied by an Mo3 cluster, the Moα-in and Moβ binding sites contain rather weak and non-uniform electron density for the Mo atoms (but clearly identifiable by anomalous data), suggesting the presence of POM cluster ensembles and/or degradation products of larger aggregates. The "Moα-in cluster ensemble" was interpreted as an antiprism-like Mo6 species superimposed with an Mo7 pyramide and the "Moβ cluster ensemble" as an Mo13 cluster (present mostly in a degraded form) composed of a pyramidal Mo7 and a Mo3 building block linked by three spatially separated MoOx units. Inside the ball-shaped Mo13 cluster sits an occluded central atom, perhaps a metal ion. POM cluster formation at the Moα-in and Moβ sites appears to be driven by filtering out and binding/protecting self-assembled transient species complementary to the protein template.

PubMed: 24945101
DOI: 10.1016/j.jinorgbio.2014.05.009

実験手法

X-RAY DIFFRACTION (1.6 Å)