4NC3

Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase.

4NC3 の概要

• エントリーDOI: 10.2210/pdb4nc3/pdb
• 関連するPDBエントリー: 4IB4
• 分子名称: Chimera protein of human 5-hydroxytryptamine receptor 2B and E. Coli soluble cytochrome b562, PALMITIC ACID, Ergotamine, ... (10 entities in total)
• 機能のキーワード: serial femtosecond crystallography, human 5ht2b receptor, ergotamine, novel protein engineering, gpcr network, membrane protein, lipidic cubic phase, psi-biology, free electron laser, structural genomics, gpcr, membrane, signaling protein, electron transport
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cell membrane ; Multi- pass membrane protein : P41595
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53259.85

構造登録者

Liu, W.,Wacker, D.,Gati, C.,Han, G.W.,James, D.,Wang, D.,Nelson, G.,Weierstall, U.,Katritch, V.,Barty, A.,Zatsepin, N.A.,Li, D.,Messerschmidt, M.,Boutet, S.,Williams, G.J.,Koglin, J.E.,Seibert, M.M.,Wang, C.,Shah, S.T.A.,Basu, S.,Fromme, R.,Kupitz, C.,Rendek, K.N.,Grotjohann, I.,Fromme, P.,Kirian, R.A.,Beyerlein, K.R.,White, T.A.,Chapman, H.N.,Caffrey, M.,Spence, J.C.H.,Stevens, R.C.,Cherezov, V.,GPCR Network (GPCR) (登録日: 2013-10-23, 公開日: 2013-12-18, 最終更新日: 2024-10-30)

主引用文献

Liu, W.,Wacker, D.,Gati, C.,Han, G.W.,James, D.,Wang, D.,Nelson, G.,Weierstall, U.,Katritch, V.,Barty, A.,Zatsepin, N.A.,Li, D.,Messerschmidt, M.,Boutet, S.,Williams, G.J.,Koglin, J.E.,Seibert, M.M.,Wang, C.,Shah, S.T.,Basu, S.,Fromme, R.,Kupitz, C.,Rendek, K.N.,Grotjohann, I.,Fromme, P.,Kirian, R.A.,Beyerlein, K.R.,White, T.A.,Chapman, H.N.,Caffrey, M.,Spence, J.C.,Stevens, R.C.,Cherezov, V.
Serial femtosecond crystallography of G protein-coupled receptors.
Science, 342:1521-1524, 2013

PubMed Abstract: X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) with individual 50-femtosecond-duration x-ray pulses to minimize radiation damage and obtained a high-resolution room-temperature structure of a human serotonin receptor using sub-10-micrometer microcrystals grown in a membrane mimetic matrix known as lipidic cubic phase. Compared with the structure solved by using traditional microcrystallography from cryo-cooled crystals of about two orders of magnitude larger volume, the room-temperature XFEL structure displays a distinct distribution of thermal motions and conformations of residues that likely more accurately represent the receptor structure and dynamics in a cellular environment.

PubMed: 24357322
DOI: 10.1126/science.1244142

実験手法

X-RAY DIFFRACTION (2.8 Å)