4N9H

Crystal structure of Transcription regulation Protein CRP

4N9H の概要

• エントリーDOI: 10.2210/pdb4n9h/pdb
• 関連するPDBエントリー: 4N9I
• 分子名称: Catabolite gene activator (2 entities in total)
• 機能のキーワード: dna binding, transcription regulator
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48001.41

構造登録者

Lee, B.J.,Seok, S.H.,Im, H.,Yoon, H.J. (登録日: 2013-10-21, 公開日: 2014-07-09, 最終更新日: 2024-11-20)

主引用文献

Seok, S.H.,Im, H.,Won, H.S.,Seo, M.D.,Lee, Y.S.,Yoon, H.J.,Cha, M.J.,Park, J.Y.,Lee, B.J.
Structures of inactive CRP species reveal the atomic details of the allosteric transition that discriminates cyclic nucleotide second messengers.
Acta Crystallogr.,Sect.D, 70:1726-1742, 2014

PubMed Abstract: The prokaryotic global transcription factor CRP has been considered to be an ideal model for in-depth study of both the allostery of the protein and the differential utilization of the homologous cyclic nucleotide second messengers cAMP and cGMP. Here, atomic details from the crystal structures of two inactive CRP species, an apo form and a cGMP-bound form, in comparison with a known active conformation, the cAMP-CRP complex, provide macroscopic and microscopic insights into CRP allostery, which is coupled to specific discrimination between the two effectors. The cAMP-induced conformational transition, including dynamic fluctuations, can be driven by the fundamental folding forces that cause water-soluble globular proteins to construct an optimized hydrophobic core, including secondary-structure formation. The observed conformational asymmetries underlie a negative cooperativity in the sequential binding of cyclic nucleotides and a stepwise manner of binding with discrimination between the effector molecules. Additionally, the finding that cGMP, which is specifically recognized in a syn conformation, induces an inhibitory conformational change, rather than a null effect, on CRP supports the intriguing possibility that cGMP signalling could be widely utilized in prokaryotes, including in aggressive inhibition of CRP-like proteins.

PubMed: 24914983
DOI: 10.1107/S139900471400724X

実験手法

X-RAY DIFFRACTION (2.2 Å)