4N8F

CcmL from Thermosynechococcus elongatus BP-1

Summary for 4N8F

• Entry DOI: 10.2210/pdb4n8f/pdb
• Related: 4N8X
• Descriptor: Carbon dioxide concentrating mechanism protein, SULFATE ION, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: ccml, stranded beta barrel, bacterial microcompartment vertex protein, carboxysome, structural protein
• Biological source: Thermosynechococcus elongatus
• Total number of polymer chains: 5
• Total formula weight: 62490.24

Authors

Kimber, M.S.,Demers, R.J. (deposition date: 2013-10-17, release date: 2014-03-12, Last modification date: 2023-09-20)

Primary citation

Keeling, T.J.,Samborska, B.,Demers, R.W.,Kimber, M.S.
Interactions and structural variability of beta-carboxysomal shell protein CcmL.
Photosynth.Res., 121:125-133, 2014

PubMed Abstract: CcmL is a small, pentameric protein that is argued to fill the vertices of β-carboxysomal shell. Here we report the structures of two CcmL orthologs, those from Nostoc sp. PCC 7120 and Thermosynechococcus elongatus BP-1. These structures broadly resemble those previously reported for other strains. However, the Nostoc CcmL structure shows an interesting pattern of behavior where two loops that map to the base of the pentamer adopt either an out or in conformation, with a consistent (over six pentamers) out-in-out-in-in pattern of protomers. The pentamers in this structure are also consistently organized into a back-to-back decamer, though evidence suggests that this is likely not present in solution. Förster resonance energy transfer experiments were able to show a weak interaction between CcmL and CcmK2 when CcmK2 was present at >100 μM. Since CcmK2 forms defined bodies with approximately 200 nm diameter at this concentration, this would support the idea that CcmL can only interact with CcmK2 at rare defect points in the growing shell.

PubMed: 24504539
DOI: 10.1007/s11120-014-9973-z

Experimental method

X-RAY DIFFRACTION (2 Å)