4N0T

Core structure of the U6 small nuclear ribonucleoprotein at 1.7 Angstrom resolution

4N0T の概要

• エントリーDOI: 10.2210/pdb4n0t/pdb
• 分子名称: U4/U6 snRNA-associated-splicing factor PRP24, U6 snRNA, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: spliceosomal ribonucleoprotein complex, annealing u6 and u4 snrna, nucleus, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: P49960
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66527.68

構造登録者

Montemayor, E.J.,Curran, E.C.,Liao, H.,Andrews, K.L.,Treba, C.N.,Butcher, S.E.,Brow, D.A. (登録日: 2013-10-02, 公開日: 2014-05-14, 最終更新日: 2023-09-20)

主引用文献

Montemayor, E.J.,Curran, E.C.,Liao, H.H.,Andrews, K.L.,Treba, C.N.,Butcher, S.E.,Brow, D.A.
Core structure of the U6 small nuclear ribonucleoprotein at 1.7- angstrom resolution.
Nat.Struct.Mol.Biol., 21:544-551, 2014

PubMed Abstract: The spliceosome is a dynamic assembly of five small nuclear ribonucleoproteins (snRNPs) that removes introns from eukaryotic pre-mRNA. U6, the most conserved of the spliceosomal small nuclear RNAs (snRNAs), participates directly in catalysis. Here, we report the crystal structure of the Saccharomyces cerevisiae U6 snRNP core containing most of the U6 snRNA and all four RRM domains of the Prp24 protein. It reveals a unique interlocked RNP architecture that sequesters the 5' splice site-binding bases of U6 snRNA. RRMs 1, 2 and 4 of Prp24 form an electropositive groove that binds double-stranded RNA and may nucleate annealing of U4 and U6 snRNAs. Substitutions in Prp24 that suppress a mutation in U6 localize to direct RNA-protein contacts. Our results provide the most comprehensive view to date of a multi-RRM protein bound to RNA and reveal striking coevolution of protein and RNA structure.

PubMed: 24837192
DOI: 10.1038/nsmb.2832

実験手法

X-RAY DIFFRACTION (1.7 Å)