4MYC

Structure of the mitochondrial ABC transporter, Atm1

4MYC の概要

• エントリーDOI: 10.2210/pdb4myc/pdb
• 関連するPDBエントリー: 4MYH
• 分子名称: Iron-sulfur clusters transporter ATM1, mitochondrial (1 entity in total)
• 機能のキーワード: abc transporter, membrane transport, mitochondrial inner membrane, transport protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Mitochondrion inner membrane ; Multi- pass membrane protein : P40416
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 201637.64

構造登録者

Srinivasan, V. (登録日: 2013-09-27, 公開日: 2014-03-26, 最終更新日: 2024-02-28)

主引用文献

Srinivasan, V.,Pierik, A.J.,Lill, R.
Crystal structures of nucleotide-free and glutathione-bound mitochondrial ABC transporter Atm1.
Science, 343:1137-1140, 2014

PubMed Abstract: The yeast mitochondrial ABC transporter Atm1, in concert with glutathione, functions in the export of a substrate required for cytosolic-nuclear iron-sulfur protein biogenesis and cellular iron regulation. Defects in the human ortholog ABCB7 cause the sideroblastic anemia XLSA/A. Here, we report the crystal structures of free and glutathione-bound Atm1 in inward-facing, open conformations at 3.06- and 3.38-angstrom resolution, respectively. The glutathione binding site includes a residue mutated in XLSA/A and is located close to the inner membrane surface in a large cavity. The two nucleotide-free adenosine 5'-triphosphate binding domains do not interact yet are kept in close vicinity through tight interaction of the two C-terminal α-helices of the Atm1 dimer. The resulting protein stabilization may be a common structural feature of all ABC exporters.

PubMed: 24604199
DOI: 10.1126/science.1246729

実験手法

X-RAY DIFFRACTION (3.06 Å)