4MW3

Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel

4MW3 の概要

• エントリーDOI: 10.2210/pdb4mw3/pdb
• 関連するPDBエントリー: 4MS2 4MTF 4MTG 4MTO 4MVM 4MVO 4MVQ 4MVR 4MVS 4MVU 4MVZ
• 分子名称: Ion transport protein, CALCIUM ION, 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total)
• 機能のキーワード: tetrameric, voltage-gated ion channel, voltage-gated calcium channel, transport protein, membrane, metal transport
• 由来する生物種: Arcobacter butzleri
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 120882.01

構造登録者

Tang, L.,Gamal El-Din, T.M.,Payandeh, J.,Martinez, G.Q.,Heard, T.M.,Scheuer, T.,Zheng, N.,Catterall, W.A. (登録日: 2013-09-24, 公開日: 2013-11-27, 最終更新日: 2024-02-28)

主引用文献

Tang, L.,Gamal El-Din, T.M.,Payandeh, J.,Martinez, G.Q.,Heard, T.M.,Scheuer, T.,Zheng, N.,Catterall, W.A.
Structural basis for Ca2+ selectivity of a voltage-gated calcium channel.
Nature, 505:56-61, 2014

PubMed Abstract: Voltage-gated calcium (CaV) channels catalyse rapid, highly selective influx of Ca(2+) into cells despite a 70-fold higher extracellular concentration of Na(+). How CaV channels solve this fundamental biophysical problem remains unclear. Here we report physiological and crystallographic analyses of a calcium selectivity filter constructed in the homotetrameric bacterial NaV channel NaVAb. Our results reveal interactions of hydrated Ca(2+) with two high-affinity Ca(2+)-binding sites followed by a third lower-affinity site that would coordinate Ca(2+) as it moves inward. At the selectivity filter entry, Site 1 is formed by four carboxyl side chains, which have a critical role in determining Ca(2+) selectivity. Four carboxyls plus four backbone carbonyls form Site 2, which is targeted by the blocking cations Cd(2+) and Mn(2+), with single occupancy. The lower-affinity Site 3 is formed by four backbone carbonyls alone, which mediate exit into the central cavity. This pore architecture suggests a conduction pathway involving transitions between two main states with one or two hydrated Ca(2+) ions bound in the selectivity filter and supports a 'knock-off' mechanism of ion permeation through a stepwise-binding process. The multi-ion selectivity filter of our CaVAb model establishes a structural framework for understanding the mechanisms of ion selectivity and conductance by vertebrate CaV channels.

PubMed: 24270805
DOI: 10.1038/nature12775

実験手法

X-RAY DIFFRACTION (3.2997 Å)