4MPM

Wild-type human neuroglobin

4MPM の概要

• エントリーDOI: 10.2210/pdb4mpm/pdb
• 関連するPDBエントリー: 1OJ6
• 分子名称: Neuroglobin, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: globin, oxygen supply, nitrite reductase, proto-porphyrin ix, brain neurons, apoptosis
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Perikaryon (By similarity): Q9NPG2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35135.72

構造登録者

Guimaraes, B.G.,Golinelli-Pimpaneau, B. (登録日: 2013-09-13, 公開日: 2014-01-15, 最終更新日: 2024-11-06)

主引用文献

Guimaraes, B.G.,Hamdane, D.,Lechauve, C.,Marden, M.C.,Golinelli-Pimpaneau, B.
The crystal structure of wild-type human brain neuroglobin reveals flexibility of the disulfide bond that regulates oxygen affinity.
Acta Crystallogr.,Sect.D, 70:1005-1014, 2014

PubMed Abstract: Neuroglobin plays an important function in the supply of oxygen in nervous tissues. In human neuroglobin, a cysteine at position 46 in the loop connecting the C and D helices of the globin fold is presumed to form an intramolecular disulfide bond with Cys55. Rupture of this disulfide bridge stabilizes bi-histidyl haem hexacoordination, causing an overall decrease in the affinity for oxygen. Here, the first X-ray structure of wild-type human neuroglobin is reported at 1.74 Å resolution. This structure provides a direct observation of two distinct conformations of the CD region containing the intramolecular disulfide link and highlights internal cavities that could be involved in ligand migration and/or are necessary to enable the conformational transition between the low and high oxygen-affinity states following S-S bond formation.

PubMed: 24699645
DOI: 10.1107/S1399004714000078

実験手法

X-RAY DIFFRACTION (1.74 Å)