4MLO

1.65A resolution structure of ToxT from Vibrio cholerae (P21 Form)

4MLO の概要

• エントリーDOI: 10.2210/pdb4mlo/pdb
• 分子名称: TCP pilus virulence regulatory protein, CHLORIDE ION, PALMITOLEIC ACID, ... (4 entities in total)
• 機能のキーワード: toxt, dna binding, transcription regulator
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32452.79

構造登録者

Lovell, S.,Wehmeyer, G.,Battaile, K.P.,Li, J.,Egan, S. (登録日: 2013-09-06, 公開日: 2016-04-20, 最終更新日: 2023-09-20)

主引用文献

Li, J.,Wehmeyer, G.,Lovell, S.,Battaile, K.P.,Egan, S.M.
1.65 angstrom resolution structure of the AraC-family transcriptional activator ToxT from Vibrio cholerae.
Acta Crystallogr F Struct Biol Commun, 72:726-731, 2016

PubMed Abstract: ToxT is an AraC-family transcriptional activator protein that controls the expression of key virulence factors in Vibrio cholerae, the causative agent of cholera. ToxT directly activates the expression of the genes that encode the toxin-coregulated pilus and cholera toxin, and also positively auto-regulates its own expression from the tcp promoter. The crystal structure of ToxT has previously been solved at 1.9 Å resolution (PDB entry 3gbg). In this study, a crystal structure of ToxT at 1.65 Å resolution with a similar overall structure to the previously determined structure is reported. However, there are distinct differences between the two structures, particularly in the region that extends from Asp101 to Glu110. This region, which can influence ToxT activity but was disordered in the previous structure, can be traced entirely in the current structure.

PubMed: 27599865
DOI: 10.1107/S2053230X1601298X

実験手法

X-RAY DIFFRACTION (1.65 Å)