4MJ0

BK Polyomavirus VP1 pentamer in complex with GD3 oligosaccharide

4MJ0 の概要

• エントリーDOI: 10.2210/pdb4mj0/pdb
• 関連するPDBエントリー: 3BWQ 3BWR 3NXD 4MJ1
• 分子名称: VP1 capsid protein, N-acetyl-alpha-neuraminic acid-(2-8)-N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, N-acetyl-alpha-neuraminic acid-(2-8)-N-acetyl-alpha-neuraminic acid, ... (7 entities in total)
• 機能のキーワード: antiparallel beta sandwich, jelly-roll topology, viral protein, polyomavirus, receptor-switching, glycan receptor, virus major capsid protein, attachment to host-cell surface receptors
• 由来する生物種: BK polyomavirus (BKPyV)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 155015.64

構造登録者

Neu, U.,Stroh, L.J.,Stehle, T. (登録日: 2013-09-03, 公開日: 2013-11-06, 最終更新日: 2024-04-03)

主引用文献

Neu, U.,Allen, S.A.,Blaum, B.S.,Liu, Y.,Frank, M.,Palma, A.S.,Stroh, L.J.,Feizi, T.,Peters, T.,Atwood, W.J.,Stehle, T.
A Structure-Guided Mutation in the Major Capsid Protein Retargets BK Polyomavirus.
Plos Pathog., 9:e1003688-e1003688, 2013

PubMed Abstract: Viruses within a family often vary in their cellular tropism and pathogenicity. In many cases, these variations are due to viruses switching their specificity from one cell surface receptor to another. The structural requirements that underlie such receptor switching are not well understood especially for carbohydrate-binding viruses, as methods capable of structure-specificity studies are only relatively recently being developed for carbohydrates. We have characterized the receptor specificity, structure and infectivity of the human polyomavirus BKPyV, the causative agent of polyomavirus-associated nephropathy, and uncover a molecular switch for binding different carbohydrate receptors. We show that the b-series gangliosides GD3, GD2, GD1b and GT1b all can serve as receptors for BKPyV. The crystal structure of the BKPyV capsid protein VP1 in complex with GD3 reveals contacts with two sialic acid moieties in the receptor, providing a basis for the observed specificity. Comparison with the structure of simian virus 40 (SV40) VP1 bound to ganglioside GM1 identifies the amino acid at position 68 as a determinant of specificity. Mutation of this residue from lysine in BKPyV to serine in SV40 switches the receptor specificity of BKPyV from GD3 to GM1 both in vitro and in cell culture. Our findings highlight the plasticity of viral receptor binding sites and form a template to retarget viruses to different receptors and cell types.

PubMed: 24130487
DOI: 10.1371/journal.ppat.1003688

実験手法

X-RAY DIFFRACTION (1.7 Å)