4MGU

Crystal structure of Acheta domesticus Densovirus

4MGU の概要

• エントリーDOI: 10.2210/pdb4mgu/pdb
• 分子名称: Structural protein VP2, DNA (5'-D(P*TP*TP*T)-3') (2 entities in total)
• 機能のキーワード: virus, jelly-roll, dnv, virus-dna complex, virus/dna
• 由来する生物種: Acheta domestica densovirus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47715.14

構造登録者

Meng, G.,Rossmann, M.G. (登録日: 2013-08-28, 公開日: 2013-10-30, 最終更新日: 2024-02-28)

主引用文献

Meng, G.,Zhang, X.,Plevka, P.,Yu, Q.,Tijssen, P.,Rossmann, M.G.
The structure and host entry of an invertebrate parvovirus.
J.Virol., 87:12523-12530, 2013

PubMed Abstract: The 3.5-Å resolution X-ray crystal structure of mature cricket parvovirus (Acheta domesticus densovirus [AdDNV]) has been determined. Structural comparisons show that vertebrate and invertebrate parvoviruses have evolved independently, although there are common structural features among all parvovirus capsid proteins. It was shown that raising the temperature of the AdDNV particles caused a loss of their genomes. The structure of these emptied particles was determined by cryo-electron microscopy to 5.5-Å resolution, and the capsid structure was found to be the same as that for the full, mature virus except for the absence of the three ordered nucleotides observed in the crystal structure. The viral protein 1 (VP1) amino termini could be externalized without significant damage to the capsid. In vitro, this externalization of the VP1 amino termini is accompanied by the release of the viral genome.

PubMed: 24027306
DOI: 10.1128/JVI.01822-13

実験手法

X-RAY DIFFRACTION (3.5 Å)