4MFV

Crystal structure of human CTNNBL1(residues 33~563)

4MFV の概要

• エントリーDOI: 10.2210/pdb4mfv/pdb
• 関連するPDBエントリー: 4MFU
• 分子名称: Beta-catenin-like protein 1 (1 entity in total)
• 機能のキーワード: arm repeats, gene regulation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Nucleus. Isoform 2: Cytoplasm : Q8WYA6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 123360.75

構造登録者

Ahn, J.W.,Kim, S.,Kim, K.J. (登録日: 2013-08-28, 公開日: 2014-03-12, 最終更新日: 2023-11-08)

主引用文献

Ahn, J.W.,Kim, S.,Kim, E.J.,Kim, Y.J.,Kim, K.J.
Structural insights into the novel ARM-repeat protein CTNNBL1 and its association with the hPrp19-CDC5L complex
Acta Crystallogr.,Sect.D, 70:780-788, 2014

PubMed Abstract: The hPrp19-CDC5L complex plays a crucial role during human pre-mRNA splicing by catalytic activation of the spliceosome. In order to elucidate the molecular architecture of the hPrp19-CDC5L complex, the crystal structure of CTNNBL1, one of the major components of this complex, was determined. Unlike canonical ARM-repeat proteins such as β-catenin and importin-α, CTNNBL1 was found to contain a twisted and extended ARM-repeat structure at the C-terminal domain and, more importantly, the protein formed a stable dimer. A highly negatively charged patch formed in the N-terminal ARM-repeat domain of CTNNBL1 provides a binding site for CDC5L, a binding partner of the protein in the hPrp19-CDC5L complex, and these two proteins form a complex with a stoichiometry of 2:2. These findings not only present the crystal structure of a novel ARM-repeat protein, CTNNBL1, but also provide insights into the detailed molecular architecture of the hPrp19-CDC5L complex.

PubMed: 24598747
DOI: 10.1107/S139900471303318X

実験手法

X-RAY DIFFRACTION (2.92 Å)