4MDX

Crystal structure of Bacillus subtilis MazF in complex with RNA

4MDX の概要

• エントリーDOI: 10.2210/pdb4mdx/pdb
• 分子名称: mRNA interferase EndoA, RNA, mRNA, IODIDE ION, ... (5 entities in total)
• 機能のキーワード: toxin-antitoxin complex, toxin-mrna complex, mazf, mrna interferase, endoa, ydce, toxin, antitoxin, maze, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Bacillus subtilis subsp. subtilis; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 29732.17

構造登録者

Simanshu, D.K.,Patel, D.J. (登録日: 2013-08-23, 公開日: 2013-10-23, 最終更新日: 2024-02-28)

主引用文献

Simanshu, D.K.,Yamaguchi, Y.,Park, J.H.,Inouye, M.,Patel, D.J.
Structural Basis of mRNA Recognition and Cleavage by Toxin MazF and Its Regulation by Antitoxin MazE in Bacillus subtilis.
Mol.Cell, 52:447-458, 2013

PubMed Abstract: MazF is an mRNA interferase, which, upon activation during stress conditions, cleaves mRNAs in a sequence-specific manner, resulting in cellular growth arrest. During normal growth conditions, the MazF toxin is inactivated through binding to its cognate antitoxin, MazE. How MazF specifically recognizes its mRNA target and carries out cleavage and how the formation of the MazE-MazF complex inactivates MazF remain unclear. We present crystal structures of MazF in complex with mRNA substrate and antitoxin MazE in Bacillus subtilis. The structure of MazF in complex with uncleavable UUdUACAUAA RNA substrate defines the molecular basis underlying the sequence-specific recognition of UACAU and the role of residues involved in the cleavage through site-specific mutational studies. The structure of the heterohexameric (MazF)2-(MazE)2-(MazF)2 complex in Bacillus subtilis, supplemented by mutational data, demonstrates that the positioning of the C-terminal helical segment of MazE within the RNA-binding channel of the MazF dimer prevents mRNA binding and cleavage by MazF.

PubMed: 24120662
DOI: 10.1016/j.molcel.2013.09.006

実験手法

X-RAY DIFFRACTION (1.5 Å)