4M8Z

Crystal Structure of SFH3, a phosphatidylinositol transfer protein that integrates phosphoinositide signaling with lipid droplet metabolism

4M8Z の概要

• エントリーDOI: 10.2210/pdb4m8z/pdb
• 分子名称: Phosphatidylinositol transfer protein PDR16 (2 entities in total)
• 機能のキーワード: lipid droplets, phosphoinositides, meiosis, cral trio, sec14, phospholipid signaling, signaling protein
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82026.84

構造登録者

Ortlund, E.O.,Pathak, M.C. (登録日: 2013-08-14, 公開日: 2014-09-10, 最終更新日: 2024-10-16)

主引用文献

Ren, J.,Pei-Chen Lin, C.,Pathak, M.C.,Temple, B.R.,Nile, A.H.,Mousley, C.J.,Duncan, M.C.,Eckert, D.M.,Leiker, T.J.,Ivanova, P.T.,Myers, D.S.,Murphy, R.C.,Brown, H.A.,Verdaasdonk, J.,Bloom, K.S.,Ortlund, E.A.,Neiman, A.M.,Bankaitis, V.A.
A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis.
Mol Biol Cell, 25:712-727, 2014

PubMed Abstract: Lipid droplet (LD) utilization is an important cellular activity that regulates energy balance and release of lipid second messengers. Because fatty acids exhibit both beneficial and toxic properties, their release from LDs must be controlled. Here we demonstrate that yeast Sfh3, an unusual Sec14-like phosphatidylinositol transfer protein, is an LD-associated protein that inhibits lipid mobilization from these particles. We further document a complex biochemical diversification of LDs during sporulation in which Sfh3 and select other LD proteins redistribute into discrete LD subpopulations. The data show that Sfh3 modulates the efficiency with which a neutral lipid hydrolase-rich LD subclass is consumed during biogenesis of specialized membrane envelopes that package replicated haploid meiotic genomes. These results present novel insights into the interface between phosphoinositide signaling and developmental regulation of LD metabolism and unveil meiosis-specific aspects of Sfh3 (and phosphoinositide) biology that are invisible to contemporary haploid-centric cell biological, proteomic, and functional genomics approaches.

PubMed: 24403601
DOI: 10.1091/mbc.E13-11-0634

実験手法

X-RAY DIFFRACTION (1.928 Å)