4M7M

Crystal structure of the R111K:R132Q:Y134F:T54V:R59W:A32W mutant of the Cellular Retinoic Acid Binding Protein Type II in complex with All-Trans Retinal at 2.57 Angstrom Resolution

4M7M の概要

• エントリーDOI: 10.2210/pdb4m7m/pdb
• 関連するPDBエントリー: 2FR3 2G78 2G7B 3F8A 3FEP 4EEJ 4EFG 4EXZ 4I9R 4I9S 4M6S
• 分子名称: Cellular retinoic acid-binding protein 2, RETINAL (3 entities in total)
• 機能のキーワード: protein engineering, wavelength regulation, ph-sensing, retinylidene psb, iminium, transport protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P29373
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15935.34

構造登録者

Nosrati, M.,Geiger, J.H. (登録日: 2013-08-12, 公開日: 2013-10-09, 最終更新日: 2024-11-27)

主引用文献

Berbasova, T.,Nosrati, M.,Vasileiou, C.,Wang, W.,Lee, K.S.,Yapici, I.,Geiger, J.H.,Borhan, B.
Rational Design of a Colorimetric pH Sensor from a Soluble Retinoic Acid Chaperone.
J.Am.Chem.Soc., 135:16111-16119, 2013

PubMed Abstract: Reengineering of cellular retinoic acid binding protein II (CRABPII) to be capable of binding retinal as a protonated Schiff base is described. Through rational alterations of the binding pocket, electrostatic perturbations of the embedded retinylidene chromophore that favor delocalization of the iminium charge lead to exquisite control in the regulation of chromophoric absorption properties, spanning the visible spectrum (474-640 nm). The pKa of the retinylidene protonated Schiff base was modulated from 2.4 to 8.1, giving rise to a set of proteins of varying colors and pH sensitivities. These proteins were used to demonstrate a concentration-independent, ratiometric pH sensor.

PubMed: 24059243
DOI: 10.1021/ja404900k

実験手法

X-RAY DIFFRACTION (2.571 Å)