4M2R

Human Carbonic Anhydrase II in complex with Brinzolamide

Summary for 4M2R

• Entry DOI: 10.2210/pdb4m2r/pdb
• Related: 4M2U 4M2V 4M2W
• Descriptor: Carbonic anhydrase 2, ZINC ION, (+)-4-ETHYLAMINO-3,4-DIHYDRO-2-(METHOXY)PROPYL-2H-THIENO[3,2-E]-1,2-THIAZINE-6-SULFONAMIDE-1,1-DIOXIDE, ... (4 entities in total)
• Functional Keywords: lyase-lyase inhibitor complex, lyase/lyase inhibitor
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm : P00918
• Total number of polymer chains: 1
• Total formula weight: 29381.56

Authors

Pinard, M.P.,Boone, C.D.,Rife, B.D.,Supuran, C.T.,Mckenna, R. (deposition date: 2013-08-05, release date: 2013-11-06, Last modification date: 2024-02-28)

Primary citation

Pinard, M.A.,Boone, C.D.,Rife, B.D.,Supuran, C.T.,McKenna, R.
Structural study of interaction between brinzolamide and dorzolamide inhibition of human carbonic anhydrases.
Bioorg.Med.Chem., 21:7210-7215, 2013

PubMed Abstract: Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes that catalyze the reversible hydration of carbon dioxide and bicarbonate. Their pivotal role in metabolism, ubiquitous nature, and multiple isoforms (CA I-XIV) has made CAs an attractive drug target in clinical applications. The usefulness of CA inhibitors (CAIs) in the treatment of glaucoma and epilepsy are well documented. In addition several isoforms of CAs (namely, CA IX) also serve as biological markers for certain tumors, and therefore they have the potential for useful applications in the treatment of cancer. This is a structural study on the binding interactions of the widely used CA inhibitory drugs brinzolamide (marketed as Azopt®) and dorzolamide (marketed as Trusopt®) with CA II and a CA IX-mimic, which was created via site-directed mutagenesis of CA II cDNA such that the active site resembles that of CA IX. Also the inhibition of CA II and CA IX and molecular docking reveal brinzolamide to be a more potent inhibitor among the other catalytically active CA isoforms compared to dorzolamide. The structures show that the tail end of the sulfonamide inhibitor is critical in forming stabilizing interactions that influence tight binding; therefore, for future drug design it is the tail moiety that will ultimately determine isoform specificity.

PubMed: 24090602
DOI: 10.1016/j.bmc.2013.08.033

Experimental method

X-RAY DIFFRACTION (1.993 Å)