4LZI

Characterization of Solanum tuberosum Multicystatin and Significance of Core Domains

4LZI の概要

• エントリーDOI: 10.2210/pdb4lzi/pdb
• 分子名称: Multicystatin (2 entities in total)
• 機能のキーワード: cystatin, hydrolase inhibitor
• 由来する生物種: Solanum tuberosum (potatoes)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32585.29

構造登録者

Nissen, M.S.,Kumar, G.N.,Green, A.R.,Knowles, N.R.,Kang, C. (登録日: 2013-07-31, 公開日: 2014-02-26, 最終更新日: 2023-09-20)

主引用文献

Green, A.R.,Nissen, M.S.,Kumar, G.N.,Knowles, N.R.,Kang, C.
Characterization of Solanum tuberosum Multicystatin and the Significance of Core Domains.
Plant Cell, 25:5043-5052, 2013

PubMed Abstract: Potato (Solanum tuberosum) multicystatin (PMC) is a unique cystatin composed of eight repeating units, each capable of inhibiting cysteine proteases. PMC is a composite of several cystatins linked by trypsin-sensitive (serine protease) domains and undergoes transitions between soluble and crystalline forms. However, the significance and the regulatory mechanism or mechanisms governing these transitions are not clearly established. Here, we report the 2.2-Å crystal structure of the trypsin-resistant PMC core consisting of the fifth, sixth, and seventh domains. The observed interdomain interaction explains PMC's resistance to trypsin and pH-dependent solubility/aggregation. Under acidic pH, weakening of the interdomain interactions exposes individual domains, resulting in not only depolymerization of the crystalline form but also exposure of cystatin domains for inhibition of cysteine proteases. This in turn allows serine protease-mediated fragmentation of PMC, producing ∼ 10-kD domains with intact inhibitory capacity and faster diffusion, thus enhancing PMC's inhibitory ability toward cysteine proteases. The crystal structure, light-scattering experiments, isothermal titration calorimetry, and site-directed mutagenesis confirmed the critical role of pH and N-terminal residues in these dynamic transitions between monomer/polymer of PMC. Our data support a notion that the pH-dependent structural regulation of PMC has defense-related implications in tuber physiology via its ability to regulate protein catabolism.

PubMed: 24363310
DOI: 10.1105/tpc.113.121004

実験手法

X-RAY DIFFRACTION (2.2 Å)