4LZ2

Crystal structure of the bromodomain of human BAZ2A

Summary for 4LZ2

• Entry DOI: 10.2210/pdb4lz2/pdb
• Descriptor: Bromodomain adjacent to zinc finger domain protein 2A, 1,2-ETHANEDIOL, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: structural genomics consortium, sgc, signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus, nucleolus : Q9UIF9
• Total number of polymer chains: 1
• Total formula weight: 12781.63

Authors

Tallant, C.,Nunez-Alonso, G.,Picaud, S.,Filippakopoulos, P.,Krojer, T.,Bradley, A.,von Delft, F.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C.,Knapp, S.,Structural Genomics Consortium (SGC) (deposition date: 2013-07-31, release date: 2013-08-21, Last modification date: 2023-09-20)

Primary citation

Tallant, C.,Valentini, E.,Fedorov, O.,Overvoorde, L.,Ferguson, F.M.,Filippakopoulos, P.,Svergun, D.I.,Knapp, S.,Ciulli, A.
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Structure, 23:80-92, 2015

PubMed Abstract: Binding of the chromatin remodeling complex NoRC to RNA complementary to the rDNA promoter mediates transcriptional repression. TIP5, the largest subunit of NoRC, is involved in recruitment to rDNA by interactions with promoter-bound TTF-I, pRNA, and acetylation of H4K16. TIP5 domains that recognize posttranslational modifications on histones are essential for recruitment of NoRC to chromatin, but how these reader modules recognize site-specific histone tails has remained elusive. Here, we report crystal structures of PHD zinc finger and bromodomains from human TIP5 and BAZ2B in free form and bound to H3 and/or H4 histones. PHD finger functions as an independent structural module in recognizing unmodified H3 histone tails, and the bromodomain prefers H3 and H4 acetylation marks followed by a key basic residue, KacXXR. Further low-resolution analyses of PHD-bromodomain modules provide molecular insights into their trans histone tail recognition, required for nucleosome recruitment and transcriptional repression of the NoRC complex.

PubMed: 25533489
DOI: 10.1016/j.str.2014.10.017

Experimental method

X-RAY DIFFRACTION (1.76 Å)