4LVP

Crystal structure of IFT81 N-terminal domain

4LVP の概要

• エントリーDOI: 10.2210/pdb4lvp/pdb
• 関連するPDBエントリー: 4LVR
• 分子名称: Intraflagellar transport protein 81, Tantalum (3 entities in total)
• 機能のキーワード: ch domain, tubulin/microtubules, transport protein
• 由来する生物種: Chlamydomonas reinhardtii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15198.53

構造登録者

Bhogaraju, S. (登録日: 2013-07-26, 公開日: 2013-09-11, 最終更新日: 2024-02-28)

主引用文献

Bhogaraju, S.,Cajanek, L.,Fort, C.,Blisnick, T.,Weber, K.,Taschner, M.,Mizuno, N.,Lamla, S.,Bastin, P.,Nigg, E.A.,Lorentzen, E.
Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81.
Science, 341:1009-1012, 2013

PubMed Abstract: Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.

PubMed: 23990561
DOI: 10.1126/science.1240985

実験手法

X-RAY DIFFRACTION (2.323 Å)