4LVH

Insight into highly conserved H1 subtype-specific epitopes in influenza virus hemagglutinin

4LVH の概要

• エントリーDOI: 10.2210/pdb4lvh/pdb
• 関連するPDBエントリー: 4EDA 4F15
• 分子名称: Hemagglutinin, MONOCLONAL ANTIBODY H-CHAIN, MONOCLONAL ANTIBODY L-CHAIN, ... (5 entities in total)
• 機能のキーワード: influenza virus, immune system, hemagglutinin, antibody
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 419572.28

構造登録者

Kim, K.H.,Cho, K.J.,Kim, S.,Seok, J.H.,Lee, J.-H. (登録日: 2013-07-26, 公開日: 2014-04-09, 最終更新日: 2024-11-13)

主引用文献

Cho, K.J.,Hong, K.W.,Kim, S.H.,Seok, J.H.,Kim, S.,Lee, J.-H.,Saelens, X.,Kim, K.H.
Insight into highly conserved h1 subtype-specific epitopes in influenza virus hemagglutinin
Plos One, 9:e89803-e89803, 2014

PubMed Abstract: Influenza viruses continuously undergo antigenic changes with gradual accumulation of mutations in hemagglutinin (HA) that is a major determinant in subtype specificity. The identification of conserved epitopes within specific HA subtypes gives an important clue for developing new vaccines and diagnostics. We produced and characterized nine monoclonal antibodies that showed significant neutralizing activities against H1 subtype influenza viruses, and determined the complex structure of HA derived from a 2009 pandemic virus A/Korea/01/2009 (KR01) and the Fab fragment from H1-specific monoclonal antibody GC0587. The overall structure of the complex was essentially identical to the previously determined KR01 HA-Fab0757 complex structure. Both Fab0587 and Fab0757 recognize readily accessible head regions of HA, revealing broadly shared and conserved antigenic determinants among H1 subtypes. The β-strands constituted by Ser110-Glu115 and Lys169-Lys170 form H1 epitopes with distinct conformations from those of H1 and H3 HA sites. In particular, Glu112, Glu115, Lys169, and Lys171 that are highly conserved among H1 subtype HAs have close contacts with HCDR3 and LCDR3. The differences between Fab0587 and Fab0757 complexes reside mainly in HCDR3 and LCDR3, providing distinct antigenic determinants specific for 1918 pdm influenza strain. Our results demonstrate a potential key neutralizing epitope important for H1 subtype specificity in influenza virus.

PubMed: 24587046
DOI: 10.1371/journal.pone.0089803

実験手法

X-RAY DIFFRACTION (2.8 Å)