4LV3

AmpC beta-lactamase in complex with (3,5-di-tert-butylphenyl) boronic acid

4LV3 の概要

• エントリーDOI: 10.2210/pdb4lv3/pdb
• 関連するPDBエントリー: 4LV0 4LV1 4LV2
• 分子名称: Beta-lactamase, (3,5-di-tert-butylphenyl)boronic acid, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: ampc beta-lactamase, class c, hydrolase, boronic acid, covalent inhibitor, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P00811
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79739.10

構造登録者

London, N.,Eidam, O.,Shoichet, B.K. (登録日: 2013-07-25, 公開日: 2014-07-30, 最終更新日: 2024-11-20)

主引用文献

London, N.,Miller, R.M.,Krishnan, S.,Uchida, K.,Irwin, J.J.,Eidam, O.,Gibold, L.,Cimermancic, P.,Bonnet, R.,Shoichet, B.K.,Taunton, J.
Covalent docking of large libraries for the discovery of chemical probes.
Nat.Chem.Biol., 10:1066-1072, 2014

PubMed Abstract: Chemical probes that form a covalent bond with a protein target often show enhanced selectivity, potency and utility for biological studies. Despite these advantages, protein-reactive compounds are usually avoided in high-throughput screening campaigns. Here we describe a general method (DOCKovalent) for screening large virtual libraries of electrophilic small molecules. We apply this method prospectively to discover reversible covalent fragments that target distinct protein nucleophiles, including the catalytic serine of AmpC β-lactamase and noncatalytic cysteines in RSK2, MSK1 and JAK3 kinases. We identify submicromolar to low-nanomolar hits with high ligand efficiency, cellular activity and selectivity, including what are to our knowledge the first reported reversible covalent inhibitors of JAK3. Crystal structures of inhibitor complexes with AmpC and RSK2 confirm the docking predictions and guide further optimization. As covalent virtual screening may have broad utility for the rapid discovery of chemical probes, we have made the method freely available through an automated web server (http://covalent.docking.org/).

PubMed: 25344815
DOI: 10.1038/nchembio.1666

実験手法

X-RAY DIFFRACTION (1.42 Å)