4LU3

The crystal structure of the human carbonic anhydrase XIV

4LU3 の概要

• エントリーDOI: 10.2210/pdb4lu3/pdb
• 分子名称: Carbonic anhydrase 14, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ZINC ION, ... (7 entities in total)
• 機能のキーワード: zinc binding, glycoprotein, membrane, lyase-lyase inhibitor complex, lyase/lyase inhibitor
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33077.71

構造登録者

Alterio, V.,De Simone, G.,Monti, S.M. (登録日: 2013-07-24, 公開日: 2014-01-01, 最終更新日: 2024-11-20)

主引用文献

Alterio, V.,Pan, P.,Parkkila, S.,Buonanno, M.,Supuran, C.T.,Monti, S.M.,De Simone, G.
The structural comparison between membrane-associated human carbonic anhydrases provides insights into drug design of selective inhibitors.
Biopolymers, 101:769-778, 2014

PubMed Abstract: Carbonic anhydrase isoform XIV (CA XIV) is the last member of the human (h) CA family discovered so far, being localized in brain, kidneys, colon, small intestine, urinary bladder, liver, and spinal cord. It has recently been described as a possible drug target for treatment of epilepsy, some retinopathies as well as some skin tumors. Human carbonic anhydrase (hCA) XIV is a membrane-associated protein consisting of an N-terminal extracellular domain, a putative transmembrane region, and a small cytoplasmic tail. In this article, we report the expression, purification, and the crystallographic structure of the entire extracellular domain of this enzyme. The analysis of the structure revealed the typical α-CA fold, in which a 10-stranded β-sheet forms the core of the molecule, while the comparison with all the other membrane associated isoforms (hCAs IV, IX, and XII) allowed to identify the diverse oligomeric arrangement and the sequence and structural differences observed in the region 127-136 as the main factors to consider in the design of selective inhibitors for each one of the membrane associated α-CAs.

PubMed: 24374484
DOI: 10.1002/bip.22456

実験手法

X-RAY DIFFRACTION (2 Å)