4LU0

Crystal structure of 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa.

4LU0 の概要

• エントリーDOI: 10.2210/pdb4lu0/pdb
• 関連するPDBエントリー: 1GV7 1STE
• 分子名称: 2-dehydro-3-deoxyphosphooctonate aldolase (2 entities in total)
• 機能のキーワード: tim alpha-beta barrel, aldolase, phophoenol pyruvate, arabinose 5 phosphate, kdo8p, transferase
• 由来する生物種: Pseudomonas aeruginosa
• 細胞内の位置: Cytoplasm : Q9ZFK4
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 128040.05

構造登録者

Asojo, O.A. (登録日: 2013-07-24, 公開日: 2013-10-09, 最終更新日: 2023-09-20)

主引用文献

Nelson, S.K.,Kelleher, A.,Robinson, G.,Reiling, S.,Asojo, O.A.
Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa.
Acta Crystallogr.,Sect.F, 69:1084-1088, 2013

PubMed Abstract: Pseudomonas aeruginosa is a major cause of opportunistic infection and is resistant to most antibiotics. As part of efforts to generate much-needed new antibiotics, structural studies of enzymes that are critical for the virulence of P. aeruginosa but are absent in mammals have been initiated. 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (KDO8Ps), also known as 2-dehydro-3-deoxyphosphooctonate aldolase, is vital for the survival and virulence of P. aeruginosa. This enzyme catalyzes a key step in the synthesis of the lipopolysaccharide (LPS) of most Gram-negative bacteria: the condensation reaction between phosphoenolpyruvate (PEP) and arabinose 5-phosphate to produce 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate (KDO8P). This step is vital for the proper synthesis and assembly of LPS and the survival of P. aeruginosa. Here, the recombinant expression, purification and crystal structure of KDO8Ps from P. aeruginosa are presented. Orthorhombic crystals were obtained by vapor diffusion in sitting drops in the presence of 1 mM phosphoenlpyruvate. The structure reveals the prototypical α/β TIM-barrel structure expected from this family of enzymes and contains a tetramer in the asymmetric unit.

PubMed: 24100553
DOI: 10.1107/S1744309113023993

実験手法

X-RAY DIFFRACTION (2.8 Å)