4LQH

High resolution structure of native frog M ferritin

4LQH の概要

• エントリーDOI: 10.2210/pdb4lqh/pdb
• 関連するPDBエントリー: 4LPJ 4LQ7 4LQJ
• 分子名称: Ferritin, middle subunit, MAGNESIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: four helix bundle, oxidoreductase, ferroxidase
• 由来する生物種: Rana catesbeiana (bullfrogs)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21409.17

構造登録者

Mangani, S.,Di Pisa, F.,Pozzi, C.,Turano, P.,Lalli, D. (登録日: 2013-07-18, 公開日: 2014-07-23, 最終更新日: 2023-09-20)

主引用文献

Pozzi, C.,Di Pisa, F.,Lalli, D.,Rosa, C.,Theil, E.,Turano, P.,Mangani, S.
Time-lapse anomalous X-ray diffraction shows how Fe(2+) substrate ions move through ferritin protein nanocages to oxidoreductase sites.
Acta Crystallogr.,Sect.D, 71:941-953, 2015

PubMed Abstract: Ferritin superfamily protein cages reversibly synthesize internal biominerals, Fe2O3·H2O. Fe(2+) and O2 (or H2O2) substrates bind at oxidoreductase sites in the cage, initiating biomineral synthesis to concentrate iron and prevent potentially toxic reactions products from Fe(2+)and O2 or H2O2 chemistry. By freezing ferritin crystals of Rana catesbeiana ferritin M (RcMf) at different time intervals after exposure to a ferrous salt, a series of high-resolution anomalous X-ray diffraction data sets were obtained that led to crystal structures that allowed the direct observation of ferrous ions entering, moving along and binding at enzyme sites in the protein cages. The ensemble of crystal structures from both aerobic and anaerobic conditions provides snapshots of the iron substrate bound at different cage locations that vary with time. The observed differential occupation of the two iron sites in the enzyme oxidoreductase centre (with Glu23 and Glu58, and with Glu58, His61 and Glu103 as ligands, respectively) and other iron-binding sites (with Glu53, His54, Glu57, Glu136 and Asp140 as ligands) reflects the approach of the Fe(2+) substrate and its progression before the enzymatic cycle 2Fe(2+) + O2 → Fe(3+)-O-O-Fe(3+) → Fe(3+)-O(H)-Fe(3+) and turnover. The crystal structures also revealed different Fe(2+) coordination compounds bound to the ion channels located at the threefold and fourfold symmetry axes of the cage.

PubMed: 25849404
DOI: 10.1107/S1399004715002333

実験手法

X-RAY DIFFRACTION (1.16 Å)