4LMY

Structure of GAS PerR-Zn-Zn

4LMY の概要

• エントリーDOI: 10.2210/pdb4lmy/pdb
• 分子名称: Peroxide stress regulator PerR, FUR family, ZINC ION (3 entities in total)
• 機能のキーワード: transcription factor, zinc binding, transcription
• 由来する生物種: Streptococcus pyogenes
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38060.37

構造登録者

Lin, C.S.,Chao, S.Y.,Nix, J.C.,Tseng, H.L.,Tsou, C.C.,Fei, C.H.,Ciou, H.S.,Jeng, U.S.,Lin, Y.S.,Chuang, W.J.,Wu, J.J.,Wang, S. (登録日: 2013-07-11, 公開日: 2014-04-02, 最終更新日: 2024-03-20)

主引用文献

Lin, C.S.,Chao, S.Y.,Hammel, M.,Nix, J.C.,Tseng, H.L.,Tsou, C.C.,Fei, C.H.,Chiou, H.S.,Jeng, U.S.,Lin, Y.S.,Chuang, W.J.,Wu, J.J.,Wang, S.
Distinct structural features of the peroxide response regulator from group a streptococcus drive DNA binding
Plos One, 9:e89027-e89027, 2014

PubMed Abstract: Group A streptococcus (GAS, Streptococcus pyogenes) is a strict human pathogen that causes severe, invasive diseases. GAS does not produce catalase, but has an ability to resist killing by reactive oxygen species (ROS) through novel mechanisms. The peroxide response regulator (PerR), a member of ferric uptake regulator (Fur) family, plays a key role for GAS to cope with oxidative stress by regulating the expression of multiple genes. Our previous studies have found that expression of an iron-binding protein, Dpr, is under the direct control of PerR. To elucidate the molecular interactions of PerR with its cognate promoter, we have carried out structural studies on PerR and PerR-DNA complex. By combining crystallography and small-angle X-ray scattering (SAXS), we confirmed that the determined PerR crystal structure reflects its conformation in solution. Through mutagenesis and biochemical analysis, we have identified DNA-binding residues suggesting that PerR binds to the dpr promoter at the per box through a winged-helix motif. Furthermore, we have performed SAXS analysis and resolved the molecular architecture of PerR-DNA complex, in which two 30 bp DNA fragments wrap around two PerR homodimers by interacting with the adjacent positively-charged winged-helix motifs. Overall, we provide structural insights into molecular recognition of DNA by PerR and define the hollow structural arrangement of PerR-30bpDNA complex, which displays a unique topology distinct from currently proposed DNA-binding models for Fur family regulators.

PubMed: 24586487
DOI: 10.1371/journal.pone.0089027

実験手法

X-RAY DIFFRACTION (1.6 Å)