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4LJZ

Crystal Structure Analysis of the E.coli holoenzyme

4LJZ の概要
エントリーDOI10.2210/pdb4ljz/pdb
分子名称DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (7 entities in total)
機能のキーワードdna directed rna polymerase, transferase
由来する生物種Escherichia coli
詳細
細胞内の位置Cytoplasm (Potential): P00579
タンパク質・核酸の鎖数12
化学式量合計859651.66
構造登録者
Bae, B.,Darst, S.A. (登録日: 2013-07-05, 公開日: 2013-11-13, 最終更新日: 2024-02-28)
主引用文献Bae, B.,Davis, E.,Brown, D.,Campbell, E.A.,Wigneshweraraj, S.,Darst, S.A.
Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of sigma 70 domain 1.1.
Proc.Natl.Acad.Sci.USA, 110:19772-19777, 2013
Cited by
PubMed Abstract: Bacteriophage T7 encodes an essential inhibitor of the Escherichia coli host RNA polymerase (RNAP), the product of gene 2 (Gp2). We determined a series of X-ray crystal structures of E. coli RNAP holoenzyme with or without Gp2. The results define the structure and location of the RNAP σ(70) subunit domain 1.1(σ(1.1)(70)) inside the RNAP active site channel, where it must be displaced by the DNA upon formation of the open promoter complex. The structures and associated data, combined with previous results, allow for a complete delineation of the mechanism for Gp2 inhibition of E. coli RNAP. In the primary inhibition mechanism, Gp2 forms a protein-protein interaction with σ(1.1)(70), preventing the normal egress of σ(1.1)(70) from the RNAP active site channel. Gp2 thus misappropriates a domain of the RNAP holoenzyme, σ(1.1)(70), to inhibit the function of the enzyme.
PubMed: 24218560
DOI: 10.1073/pnas.1314576110
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (3.5871 Å)
構造検証レポート
Validation report summary of 4ljz
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-06に公開中

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