4LIX

Crystal structure of ent-copalyl diphosphate synthase from Arabidopsis thaliana in complex with (S)-15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate at 1.55 A resolution

Summary for 4LIX

• Entry DOI: 10.2210/pdb4lix/pdb
• Related: 3PYA 3PYB
• Descriptor: Ent-copalyl diphosphate synthase, chloroplastic, S-[(2E,6E,10E)-14-(dimethylamino)-3,7,11-trimethyltetradeca-2,6,10-trien-1-yl] trihydrogen thiodiphosphate, PHOSPHATE ION, ... (5 entities in total)
• Functional Keywords: class i and ii terpene cyclase fold, class ii diterpene cyclase, dxdd motif, gibberellin biosynthesis, biosynthesis of ent-copalyl diphosphate, isomerase
• Biological source: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• Cellular location: Plastid, chloroplast: Q38802
• Total number of polymer chains: 1
• Total formula weight: 85512.84

Authors

Koksal, M.,Christianson, D.W. (deposition date: 2013-07-04, release date: 2013-10-02, Last modification date: 2024-11-06)

Primary citation

Koksal, M.,Potter, K.,Peters, R.J.,Christianson, D.W.
1.55 angstrom -resolution structure of ent-copalyl diphosphate synthase and exploration of general acid function by site-directed mutagenesis.
Biochim.Biophys.Acta, 1840:184-190, 2013

PubMed Abstract: The diterpene cyclase ent-copalyl diphosphate synthase (CPS) catalyzes the first committed step in the biosynthesis of gibberellins. The previously reported 2.25Å resolution crystal structure of CPS complexed with (S)-15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate (1) established the αβγ domain architecture, but ambiguities regarding substrate analog binding remained.

PubMed: 24036329
DOI: 10.1016/j.bbagen.2013.09.004

Experimental method

X-RAY DIFFRACTION (1.548 Å)