4LH3

Structure of mouse 1-Pyrroline-5-Carboxylate Dehydrogenase (ALDH4A1) complexed with glutarate

4LH3 の概要

• エントリーDOI: 10.2210/pdb4lh3/pdb
• 関連するPDBエントリー: 3V9G 3V9H 3V9I 3V9J 3V9K 3V9L 4lgz 4LH0 4LH1 4LH2
• 分子名称: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, PENTAETHYLENE GLYCOL, GLUTARIC ACID, ... (4 entities in total)
• 機能のキーワード: proline catabolism substrate recognition, rossmann fold, oxidoreductase
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Mitochondrion matrix (By similarity): Q8CHT0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 124649.22

構造登録者

Pemberton, T.A.,Tanner, J.J. (登録日: 2013-06-30, 公開日: 2013-08-28, 最終更新日: 2024-02-28)

主引用文献

Pemberton, T.A.,Tanner, J.J.
Structural basis of substrate selectivity of Delta (1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1): Semialdehyde chain length.
Arch.Biochem.Biophys., 538:34-40, 2013

PubMed Abstract: The enzyme Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase (aka P5CDH and ALDH4A1) is an aldehyde dehydrogenase that catalyzes the oxidation of γ-glutamate semialdehyde to l-glutamate. The crystal structures of mouse P5CDH complexed with glutarate, succinate, malonate, glyoxylate, and acetate are reported. The structures are used to build a structure-activity relationship that describes the semialdehyde carbon chain length and the position of the aldehyde group in relation to the cysteine nucleophile and oxyanion hole. Efficient 4- and 5-carbon substrates share the common feature of being long enough to span the distance between the anchor loop at the bottom of the active site and the oxyanion hole at the top of the active site. The inactive 2- and 3-carbon semialdehydes bind the anchor loop but are too short to reach the oxyanion hole. Inhibition of P5CDH by glyoxylate, malonate, succinate, glutarate, and l-glutamate is also examined. The Ki values are 0.27 mM for glyoxylate, 58 mM for succinate, 30 mM for glutarate, and 12 mM for l-glutamate. Curiously, malonate is not an inhibitor. The trends in Ki likely reflect a trade-off between the penalty for desolvating the carboxylates of the free inhibitor and the number of compensating hydrogen bonds formed in the enzyme-inhibitor complex.

PubMed: 23928095
DOI: 10.1016/j.abb.2013.07.024

実験手法

X-RAY DIFFRACTION (1.813 Å)