4LGN

The structure of Acidothermus cellulolyticus family 74 glycoside hydrolase

4LGN の概要

• エントリーDOI: 10.2210/pdb4lgn/pdb
• 分子名称: Cellulose-binding, family II, POTASSIUM ION, 1,2-ETHANEDIOL, ... (8 entities in total)
• 機能のキーワード: gh74, xyloglucanase, cellulose binding protein
• 由来する生物種: Acidothermus cellulolyticus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 81456.34

構造登録者

Alahuhta, P.M.,Lunin, V.V. (登録日: 2013-06-28, 公開日: 2013-12-11, 最終更新日: 2023-09-20)

主引用文献

Alahuhta, M.,Adney, W.S.,Himmel, M.E.,Lunin, V.V.
Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 angstrom resolution.
Acta Crystallogr.,Sect.F, 69:1335-1338, 2013

PubMed Abstract: Here, a 1.82 Å resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an Rfree of 0.196. Structural analysis shows that five related structures have been reported with a secondary-structure similarity of between 75 and 89%. The five similar structures were all either Clostridium thermocellum or Geotrichum sp. M128 GH74 xyloglucanases. Structural analysis indicates that the A. cellulolyticus GH74 enzyme is an endoxyloglucanase, as it lacks a characteristic loop that blocks one end of the active site in exoxyloglucanases. Superimposition with the C. thermocellum GH74 shows that Asp451 and Asp38 are the catalytic residues.

PubMed: 24316824
DOI: 10.1107/S1744309113030005

実験手法

X-RAY DIFFRACTION (1.82 Å)