4LFH

Crystal Structure of 9C2 TCR

Summary for 4LFH

• Entry DOI: 10.2210/pdb4lfh/pdb
• Related: 4LHU
• Descriptor: 9C2 TCR delta chain, 9C2 TCR gamma chain, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: nkt cells, gammadelta tcr, cd1d, lipid recognition, pbs-44, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 55471.76

Authors

Uldrich, A.P.,Le Nours, J.,Pellicci, D.G.,Gras, S.,Rossjohn, J.,Godfrey, D.I. (deposition date: 2013-06-26, release date: 2013-10-02, Last modification date: 2024-11-27)

Primary citation

Uldrich, A.P.,Le Nours, J.,Pellicci, D.G.,Gherardin, N.A.,McPherson, K.G.,Lim, R.T.,Patel, O.,Beddoe, T.,Gras, S.,Rossjohn, J.,Godfrey, D.I.
CD1d-lipid antigen recognition by the gamma delta TCR.
Nat.Immunol., 14:1137-1145, 2013

PubMed Abstract: The T cell repertoire comprises αβ and γδ T cell lineages. Although it is established how αβ T cell antigen receptors (TCRs) interact with antigen presented by antigen-presenting molecules, this is unknown for γδ TCRs. We describe a population of human Vδ1(+) γδ T cells that exhibit autoreactivity to CD1d and provide a molecular basis for how a γδ TCR binds CD1d-α-galactosylceramide (α-GalCer). The γδ TCR docked orthogonally, over the A' pocket of CD1d, in which the Vδ1-chain, and in particular the germ line-encoded CDR1δ loop, dominated interactions with CD1d. The TCR γ-chain sat peripherally to the interface, with the CDR3γ loop representing the principal determinant for α-GalCer specificity. Accordingly, we provide insight into how a γδ TCR binds specifically to a lipid-loaded antigen-presenting molecule.

PubMed: 24076636
DOI: 10.1038/ni.2713

Experimental method

X-RAY DIFFRACTION (2.3 Å)