4L8L

Crystal Structure of the Type II Dehydroquinase from Pseudomonas aeruginosa

4L8L の概要

• エントリーDOI: 10.2210/pdb4l8l/pdb
• 分子名称: 3-dehydroquinate dehydratase 1 (2 entities in total)
• 機能のキーワード: flavodoxin-like fold, rossmann fold, alpha helix, beta sheet, dehydratase, dehydroquinic acid, lyase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15985.02

構造登録者

Reiling, S.A.,Asojo, O.A. (登録日: 2013-06-17, 公開日: 2014-07-23, 最終更新日: 2024-02-28)

主引用文献

Reiling, S.,Kelleher, A.,Matsumoto, M.M.,Robinson, G.,Asojo, O.A.
Structure of type II dehydroquinase from Pseudomonas aeruginosa.
Acta Crystallogr F Struct Biol Commun, 70:1485-1491, 2014

PubMed Abstract: Pseudomonas aeruginosa causes opportunistic infections and is resistant to most antibiotics. Ongoing efforts to generate much-needed new antibiotics include targeting enzymes that are vital for P. aeruginosa but are absent in mammals. One such enzyme, type II dehydroquinase (DHQase), catalyzes the interconversion of 3-dehydroquinate and 3-dehydroshikimate, a necessary step in the shikimate pathway. This step is vital for the proper synthesis of phenylalanine, tryptophan, tyrosine and other aromatic metabolites. The recombinant expression, purification and crystal structure of catalytically active DHQase from P. aeruginosa (PaDHQase) are presented. Cubic crystals belonging to space group F23, with unit-cell parameters a=b=c=125.39 Å, were obtained by vapor diffusion in sitting drops and the structure was refined to an R factor of 16% at 1.74 Å resolution. PaDHQase is a prototypical type II DHQase with the classical flavodoxin-like α/β topology.

PubMed: 25372814
DOI: 10.1107/S2053230X14020214

実験手法

X-RAY DIFFRACTION (1.74 Å)